Abstract
To determine whether vitreous lactate concentrations are correlated with intraocular pressure (IOP) rise, retinal ganglion cell (RGC) damage, and nerve fiber layer (NFL) thickness decrease in a rabbit model of ocular hypertension. Also, to learn whether proton nuclear magnetic resonance ((1)H-NMR) spectroscopy can provide sequential, non-invasive in vivo measurements of vitreous lactate. Intra-anterior chamber injections of 20-mum latex beads were used to impede aqueous drainage in New Zealand White rabbits, causing an elevation of IOP. Group I consisted of 12 rabbits in which unilateral elevations in IOP were achieved. Group II consisted of 6 rabbits in which treatment did not cause a change in IOP. The contralateral eye served as a control in both groups. Control eyes received an equal volume injection of vehicle only. IOP was measured for two pre-treatment days and then on post-treatment days 1, 3, 5, 7, 9, 16, 23, 30, and 37. (1)H-NMR spectroscopy was used to measure changes in vitreous lactate concentrations that may be associated with the onset and progression of the pathophysiology. Post-mortem histochemical analysis at the light microscope level was used to quantify changes in the retinal NFL thickness and in the number...Continue Reading
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