Longitudinal study of extended-spectrum-β-lactamase- and AmpC-producing Enterobacteriaceae in household dogs

Antimicrobial Agents and Chemotherapy
Valérie O BaedeJoost Hordijk

Abstract

A longitudinal study was performed to (i) investigate the continuity of shedding of extended-spectrum-beta-lactamase (ESBL)-producing Enterobacteriaceae in dogs without clinical signs, (ii) identify dominant plasmid-mediated ESBL genes, and (iii) quantify ESBL-producing Enterobacteriaceae in feces. Fecal samples from 38 dogs were collected monthly for 6 months. Additional samples were collected from 7 included dogs on a weekly basis for 6 weeks. Numbers of CFU per gram of feces for non-wild-type Enterobacteriaceae were determined by using MacConkey agar supplemented with 1 mg/liter cefotaxime (MCC), and those for total Enterobacteriaceae were determined by using MacConkey agar. Cefotaxime-resistant isolates were screened by PCR and sequence analysis for the presence of bla(CTX-M), bla(CMY), bla(SHV), bla(OXA), and bla(TEM) gene families. Bacterial species were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. PCR-negative isolates were tested by a double-disk synergy test for enhanced AmpC expression. A total of 259 samples were screened, and 126 samples were culture positive on MCC, resulting in 352 isolates, 327 of which were Escherichia coli. Nine dogs were co...Continue Reading

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Citations

Oct 24, 2015·Veterinary Microbiology·Carmen Espinosa-GongoraLuca Guardabassi
Nov 27, 2015·Acta Veterinaria Scandinavica·Peter DamborgLuca Guardabassi
May 2, 2016·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Massilia YousfiChristophe De Champs
Jan 13, 2018·The Veterinary Record·Freek P J van BreePaul A M Overgaauw
Apr 27, 2019·The Journal of Small Animal Practice·R H DaviesA D Wales
Nov 23, 2017·The Journal of Antimicrobial Chemotherapy·Alejandro Dorado-GarcíaDick J J Heederik
Jul 12, 2018·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Thomas GrönthalMerja Rantala
Feb 13, 2019·The Journal of Antimicrobial Chemotherapy·Joost HordijkArjan Stegeman
Nov 14, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Ricardo Antonio Pilegi SfaciotteSandra Maria Ferraz

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