Longitudinal transcriptomic characterization of the immune response to acute hepatitis C virus infection in patients with spontaneous viral clearance

PLoS Pathogens
Brad R RosenbergNaglaa H Shoukry

Abstract

Most individuals exposed to hepatitis C virus (HCV) become persistently infected while a minority spontaneously eliminate the virus. Although early immune events influence infection outcome, the cellular composition, molecular effectors, and timeframe of the host response active shortly after viral exposure remain incompletely understood. Employing specimens collected from people who inject drugs (PWID) with high risk of HCV exposure, we utilized RNA-Seq and blood transcriptome module (BTM) analysis to characterize immune function in peripheral blood mononuclear cells (PBMC) before, during, and after acute HCV infection resulting in spontaneous resolution. Our results provide a detailed description of innate immune programs active in peripheral blood during acute HCV infection, which include prominent type I interferon and inflammatory signatures. Innate immune gene expression rapidly returns to pre-infection levels upon viral clearance. Comparative analyses using peripheral blood gene expression profiles from other viral and vaccine studies demonstrate similarities in the immune responses to acute HCV and flaviviruses. Of note, both acute dengue virus (DENV) infection and acute HCV infection elicit similar innate antiviral sig...Continue Reading

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Citations

Jun 19, 2019·Journal of Clinical Medicine·Óscar Brochado-KithAmanda Fernández-Rodríguez
Nov 20, 2020·Journal of Virology·Yung-Ju YehJu-Chien Cheng
Nov 7, 2019·Microbial Pathogenesis·Lisheng ZhouXiaomin Fang
Nov 3, 2020·Current Opinion in Virology·Johnasha D StuartArash Grakoui
Apr 1, 2021·Journal of Biomedical Science·Óscar BrochadoUNKNOWN GESIDA Study Group
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Aug 31, 2021·Frontiers in Immunology·Samaa T GobranNaglaa H Shoukry

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Methods Mentioned

BETA
biopsies
RNA-Seq
PCA
biopsy
flow cytometry

Software Mentioned

CAMERA
edgeR package
ROAST
count
HTSeq
FlowJo
GSEA
MROAST
Ensembl
FACSDiva

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