Lonicera caerulea Extract Attenuates Non-Alcoholic Fatty Liver Disease in Free Fatty Acid-Induced HepG2 Hepatocytes and in High Fat Diet-Fed Mice

Nutrients
Miey ParkHae-Jeung Lee

Abstract

Honeyberry (Lonicera caerulea) has been used for medicinal purposes for thousands of years. Its predominant anthocyanin, cyanidin-3-O-glucoside (C3G), possesses antioxidant and many other potent biological activities. We aimed to investigate the effects of honeyberry extract (HBE) supplementation on HepG2 cellular steatosis induced by free fatty acids (FFA) and in diet-induced obese mice. HepG2 cells were incubated with 1 mM FFA to induce lipid accumulation with or without HBE. Obesity in mice was induced by a 45% high fat diet (HFD) for 6 weeks and subsequent supplementation of 0.5% HBE (LH) and 1% HBE (MH) for 6 weeks. HBE suppressed fatty acid synthesis and ameliorated lipid accumulation in HepG2 cells induced by FFA. Moreover, HBE also decreased lipid accumulation in the liver in the supplemented HBE group (LH, 0.5% or MH, 1%) compared with the control group. The expressions of adipogenic genes involved in hepatic lipid metabolism of sterol regulatory element-binding protein-1 (SREBP-1c), CCAAT/enhancer-binding protein alpha (C/EBPα), peroxisome proliferator-activated receptor gamma (PPARγ), and fatty acid synthase (FAS) were decreased both in the HepG2 cells and in the livers of HBE-supplemented mice. In addition, HBE incr...Continue Reading

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
May 17, 2008·The Journal of Biological Chemistry·Xiuyun HouMengwei Zang
Feb 12, 2009·Journal of Gastroenterology and Hepatology·Matteo RicchiPaola Loria
May 7, 2009·Cell Metabolism·Bei B ZhangCai Li
Dec 31, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Elisa FabbriniSamuel Klein
Oct 1, 2010·The New England Journal of Medicine·Giovanni TargherEnzo Bonora
Feb 24, 2012·The American Journal of Clinical Nutrition·Nicole M WedickRob M van Dam
Mar 1, 2012·Biochimica Et Biophysica Acta·Paul A Watkins, Jessica M Ellis
Sep 18, 2012·Current Drug Metabolism·Stéphane SavaryGérard Lizard
Dec 22, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ileana HernándezMaría Concepción Gutiérrez-Ruiz
Apr 29, 2015·Journal of Hepatology·Christopher D Byrne, Giovanni Targher
Jun 27, 2015·Oxidative Medicine and Cellular Longevity·Dumitriţa RuginăAdela Pintea
Dec 4, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu-Jie ZhangHua-Bin Li
May 26, 2016·Phytotherapy Research : PTR·Antonella SmeriglioDomenico Trombetta
Oct 18, 2016·Current Opinion in Clinical Nutrition and Metabolic Care·Katarzyna Kowalska, Anna Olejnik
Oct 25, 2016·Toxicology Letters·Yasukazu TakanezawaMasako Kiyono
Jun 18, 2017·Nutrients·Tamara Y Forbes-HernándezMaurizio Battino
Jun 18, 2017·Molecular Cell·Daniel Garcia, Reuben J Shaw

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Citations

Jul 14, 2020·Bioscience, Biotechnology, and Biochemistry·Yue GaoWenxiang Huang
Mar 16, 2021·Clinical and Experimental Pharmacology & Physiology·Ning ZhangBo Qin
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiaqi LiuSongli Shi
Jul 3, 2021·International Journal of Molecular Sciences·Maciej DanielewskiTomasz Sozański

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BETA
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PCR
ELISA
MDA

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GraphPad
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