Looking for human glandular kallikrein-1 in the prostate.

The Prostate
G ParadisJ Y Dubé

Abstract

Based on recent studies indicating that human glandular kallikrein-1 (hGK-1) mRNA was present in the prostate, we have undertaken to determine whether the prostate contained trypsin-like proteases with properties compatible with those deduced from hGK-1 gene nucleotide sequence. The first series of experiments showed that only minimal levels of trypsin-like enzymatic activity, determined with synthetic substrates, were present in chromatographic fractions of prostatic glycoproteins having a molecular weight in the range expected for hGK-1, i.e., 25,000-35,000. Because of this, we used [3H]diisopropylfluorophosphate (DFP) labeling alone or in the presence of various serine-protease inhibitors to identify trypsin-like proteases in the prostate. Two-dimensional gel electrophoresis of prostatic glycoproteins showed the presence of minor spots of 18-32 kDa. These spots were slightly more acidic than were those of prostate specific antigen (PSA) and were completely inhibited by preincubation with tosyl lysine chloromethyl ketone and p-nitrophenyl-p-guanidobenzoate in contrast to PSA spots that were not affected by these treatments. When a similar procedure was applied to total cytosolic proteins instead of glycoproteins, an additiona...Continue Reading

Citations

Dec 14, 1995·Biochimica Et Biophysica Acta·D DeperthesJ Y Dubé
Jun 1, 1991·International Journal of Andrology·G FrenetteJ Y Dubé
Feb 15, 1994·European Journal of Biochemistry·A Christensson, H Lilja
Sep 1, 1994·Clinical and Experimental Pharmacology & Physiology·J ReidB J Morris
Mar 1, 1995·Microscopy Research and Technique·M J Wilson
Oct 6, 1998·Critical Reviews in Clinical Laboratory Sciences·H G RittenhouseA W Partin

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