Loop deletions indicate regions important for FhuA transport and receptor functions in Escherichia coli

Journal of Bacteriology
Franziska Endriss, Volkmar Braun

Abstract

Precise deletions of cell surface-exposed loops of FhuA resulted in mutants of Escherichia coli with distinct phenotypes. Deletion of loop 3 or 11 inactivated ferrichrome transport activity. Deletion of loop 8 inactivated receptor activity for colicin M and the phages T1, T5, and phi80. The loop 7 deletion mutant was colicin M resistant but fully phage sensitive. The loop 4 deletion mutant was resistant to the TonB-dependent phages T1 and phi80 but fully sensitive to the TonB-independent phage T5. The phenotypes of the deletion mutants revealed important sites for the multiple FhuA transport and receptor activities. The ligand binding sites are nonidentical and are distributed among the entire exposed surface. Presumably, FhuA evolved as a ferrichrome transporter and was subsequently used as a receptor by the phages and colicin M, which selected the same as well as distinct loops as receptor sites.

References

Dec 30, 1975·Annals of the New York Academy of Sciences·H R KabackS A Short
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Jan 14, 1999·Nature Structural Biology·S K BuchananJ Deisenhofer
Jun 13, 2000·Protein Science : a Publication of the Protein Society·A D FergusonW Welte
Mar 30, 2001·The Journal of Biological Chemistry·D C ScottP E Klebba
May 10, 2001·Journal of Bacteriology·H KillmannV Braun
Mar 2, 2002·Science·Andrew D FergusonJohann Deisenhofer
Apr 6, 2002·Current Opinion in Microbiology·Volkmar Braun, Michael Braun
Nov 13, 2002·Microbiology·Helmut KillmannVolkmar Braun
Mar 26, 2003·Nature Structural Biology·David P ChimentoMichael C Wiener
Aug 2, 2003·Molecular Microbiology·Kathleen Postle, Robert J Kadner
Aug 5, 2003·Journal of Bacteriology·Franziska EndrissVolkmar Braun
Sep 2, 2003·Journal of Bacteriology·Michael BraunVolkmar Braun
Sep 6, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Volkmar Braun
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Hiroshi Nikaido

❮ Previous
Next ❯

Citations

Jul 27, 2007·Natural Product Reports·Sophie DuquesneSylvie Rebuffat
Apr 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Manuela DeziDaniel Lévy
Dec 30, 2010·The Journal of Biological Chemistry·Mohammad M MohammadLiviu Movileanu
Aug 7, 2007·Genome Research·Lise PetersenRasmus Nielsen
Mar 31, 2009·Journal of Bacteriology·Volkmar Braun
May 29, 2007·Journal of Bacteriology·Wolfgang RabschPhillip E Klebba
Sep 15, 2009·Journal of Molecular Biology·James GumbartEmad Tajkhorshid
Nov 10, 2007·Environmental Microbiology·Timothy M CarltonClive W Ronson
Nov 28, 2012·Biochemical Society Transactions·Volkmar BraunSilke I Patzer
Nov 28, 2012·Biochemical Society Transactions·Indran Mathavan, Konstantinos Beis
Apr 22, 2014·Biochimica Et Biophysica Acta·Young Chan KimChristopher N Penfold
Mar 7, 2015·Virology·Sherwood R Casjens, Roger W Hendrix
Oct 6, 2007·Biochimica Et Biophysica Acta·Karla D Krewulak, Hans J Vogel
Aug 28, 2018·Journal of Basic Microbiology·Ping LiJingxue Wang
Aug 29, 2018·Infection and Immunity·Yejun WangWolfgang Köster
May 24, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Volkmar BraunAnnette Sauter
Dec 8, 2005·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Volkmar BraunAnnette Sauter
Apr 4, 2020·Journal of Biomolecular Structure & Dynamics·Zhenhua LiDong-Qing Wei
Jul 27, 2012·Natural Product Reports·Mikhail O MaksimovA James Link

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Transport Proteins

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Bacterial Transport Proteins (ASM)

Bacterial transport proteins facilitate active and passive transport of small molecules and solutes across the bacterial membrane. Here is the latest research.