Loop extrusion: theory meets single-molecule experiments

Current Opinion in Cell Biology
Edward J Banigan, L. A. Mirny

Abstract

Chromosomes are organized as chromatin loops that promote segregation, enhancer-promoter interactions, and other genomic functions. Loops were hypothesized to form by 'loop extrusion,' by which structural maintenance of chromosomes (SMC) complexes, such as condensin and cohesin, bind to chromatin, reel it in, and extrude it as a loop. However, such exotic motor activity had never been observed. Following an explosion of indirect evidence, recent single-molecule experiments directly imaged DNA loop extrusion by condensin and cohesin in vitro. These experiments observe rapid (kb/s) extrusion that requires ATP hydrolysis and stalls under pN forces. Surprisingly, condensin extrudes loops asymmetrically, challenging previous models. Extrusion by cohesin is symmetric but requires the protein Nipbl. We discuss how SMC complexes may perform their functions on chromatin in vivo.

Citations

Sep 24, 2020·Biochemical Society Transactions·Roger Solé-Soler, Jordi Torres-Rosell
Nov 26, 2020·Essays in Biochemistry·Gurleen Kaur, Lisanne M Spenkelink
Dec 10, 2020·ELife·Edward J Banigan, Leonid A Mirny
Oct 31, 2020·Journal of Molecular Cell Biology·Qiang Wu, Jia Shou
Nov 18, 2020·Nature Communications·Cyril Matthey-DoretAxel Cournac
Jan 6, 2021·Nature Communications·Sergey V UlianovSergey V Razin
Feb 15, 2021·The FEBS Journal·Andrea M ChiarielloMario Nicodemi
Mar 28, 2021·Life Science Alliance·Julieta RivosecchiVincent Vanoosthuyse
Apr 11, 2021·Seminars in Cell & Developmental Biology·James R PaulsonWilliam C Earnshaw
Dec 1, 2020·Current Opinion in Genetics & Development·Marco Di StefanoMarc A Marti-Renom
May 1, 2021·Physical Review Letters·Kuang LiuJ M Schwarz
May 5, 2021·The Journal of Chemical Physics·Dmitry StarkovSergey Belan
Jun 11, 2021·Current Opinion in Neurobiology·Vishnu Dileep, Li-Huei Tsai
Jul 21, 2021·Biochemical Society Transactions·Andrea EspositoAndrea M Chiariello
Jul 28, 2021·Nature Structural & Molecular Biology·Hugo B BrandãoXindan Wang
Jul 22, 2021·Bioinformatics·Weibing WangYong Gao
Jul 31, 2020·Biophysical Journal·Mario Nicodemi, Simona Bianco
Sep 3, 2021·Nature Communications·Marcin J SzafranDagmara Jakimowicz
Sep 3, 2021·Nature Communications·Virginia S LioyStéphanie Bury-Moné
Jul 7, 2021·Annual Review of Cell and Developmental Biology·Rajarshi P Ghosh, Barbara J Meyer
Dec 7, 2021·PLoS Computational Biology·Lei LiuChangbong Hyeon

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