Loop-Mediated Isothermal Amplification as a Fast Noninvasive Method of Helicobacter pylori Diagnosis

Journal of Clinical Laboratory Analysis
Farideh YariAmirhooshang Alvandi

Abstract

Helicobacter pylori infection is etiologically associated with some important health problems such as gastric cancer. Because of the high clinical importance of H. pylori infection, development of a noninvasive test for the detection of H. pylori is desirable. In this study, a loop-mediated isothermal amplification (LAMP) targeted ureC of H. pylori was evaluated on 100 stool specimens and compared with a stool antigen test. Culture and rapid urease test were considered as gold standards. The overall detection rate of the fecal antigen test and LAMP was 58% and 82%, respectively. The analytical sensitivity of the fecal antigen test and LAMP was 500 and 10 H. pylori cells/g and 10 fg DNA/reaction, which is equal to six H. pylori genome. LAMP technique has been characterized by high sensitivity and low detection limit for the detection of H. pylori in stool specimen. Clinical diagnostic performance of LAMP was better than the stool antigen test.

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Citations

Aug 18, 2016·Helicobacter·Francis MégraudPhilippe Lehours
Jun 29, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Rongdan LuJunming Guo
Oct 7, 2017·Journal of Clinical Laboratory Analysis·Yongfu ShaoGuoliang Ye
Sep 12, 2017·Helicobacter·Emilie BessèdeLuiz Gonzaga Coelho
Jun 3, 2021·Journal of Clinical Medicine·Maria Pina Dore, Giovanni Mario Pes

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