Abstract
Bacterial chondronecrosis with osteomyelitis (BCO) is a major cause of lameness in broiler chicken, and results in serious economic losses worldwide. Although the pathogenesis mechanism leading to lameness is not entirely understood, some strains of Enterococcussp., avian pathogenic Escherichia coli or Staphylococcus aureus have been long recognized as important causative pathogens. To prevent the progression of Enterococcussp., avian pathogenic E. coli or S. aureus infections, we developed rapid, sensitive and convenient diagnostic assays using loop-mediated isothermal amplification (LAMP). Entero-Common-LAMP assays were developed for simultaneous detection of eight Enterococcus species. To target specific microorganisms, seven Entero-Specific-LAMP assays for E. faecalis, E. faecium, E. hirae, E. gallinarum, E. avium, E. duransand E. cecorum were developed, as well as E. coli-LAMP and S. aureus-LAMP assays. Considering the prevalence and economic impact of Enterococcussp., E. coli and S. aureus, the 10 different LAMP assays which were developed have considerable potential as routine diagnostic methods in the field or in resource-limited environments.
References
Aug 29, 1992·The Veterinary Record·S C KestinN G Gregory
Dec 1, 1972·Australian Veterinary Journal·M E Nairn, A R Watson
Mar 1, 1981·Poultry Science·E A SauterR C Stroh
Jul 1, 1980·British Poultry Science·J K ThompsonJ T Patterson
Dec 16, 1998·Veterinary Microbiology·J J McCullaghH J Ball
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Jul 30, 2002·Molecular and Cellular Probes·K NagamineT Notomi
Nov 30, 2004·The Lancet Infectious Diseases·Remco P H PetersChristina M J E Vandenbroucke-Grauls
Sep 9, 2005·Avian Pathology : Journal of the W.V.P.A·P T McNameeJ A Smyth
Dec 20, 2005·British Poultry Science·D C Deeming
Aug 1, 2000·Avian Pathology : Journal of the W.V.P.A·P T McNamee, J A Smyth
Mar 26, 2009·BioTechniques·Motoki GotoKen-Ichi Hanaki
Jul 29, 2009·British Poultry Science·I Dinev
Jul 14, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Margaret J StalkerDurda Slavic
Jan 29, 2011·Veterinary Pathology·Ø KolbjørnsenM Gilhuus
Nov 24, 2011·Avian Pathology : Journal of the W.V.P.A·M J Kense, W J M Landman
Mar 9, 2012·Poultry Science·R F WidemanI Pevzner
Feb 16, 2013·Journal of Microbiology and Biotechnology·Xihong ZhaoDeog-Hwan Oh
Mar 20, 2013·BioMed Research International·King Ting LimKwai Lin Thong
Oct 29, 2014·Applied Biochemistry and Biotechnology·Xin-Ru WangHong-Ling Ou
Dec 30, 2014·BMC Veterinary Research·Arne Jung, Silke Rautenschlein
Apr 17, 2015·PloS One·Tieshan JiangYoung Min Kwon
Nov 4, 2015·Poultry Science·Robert F Wideman
Nov 3, 2016·Avian Pathology : Journal of the W.V.P.A·Juliana Fortes Vilarinho BragaRoselene Ecco
Jun 8, 2017·Water Research·Roland MartzyGeorg H Reischer
Jul 4, 2017·Avian Pathology : Journal of the W.V.P.A·Dinidu S WijesurendraAndrew Stent