Loop-mediated isothermal amplification for rapid identification of biotypes B and Q of the globally invasive pest Bemisia tabaci, and studying population dynamics.

Pest Management Science
Chia-Hung HsiehChiun-Cheng Ko

Abstract

Bemisia tabaci, the sweetpotato whitefly, is a globally invasive pest that causes serious agricultural damage by transmitting plant viruses. This pest forms a cryptic species complex that displays morphologically indistinguishable biotypes. Among them, the B and Q biotypes are the most important pests worldwide. Because they have different levels of insecticide resistance, these biotypes must be identified in order to achieve proper pest control. Therefore, a convenient, rapid and specific detection method for identifying the two biotypes is necessary. Loop-mediated isothermal amplification (LAMP) was employed for rapid identification of B. tabaci B and Q biotypes. By combining a quick DNA extraction method, identification of the two biotypes was achieved within 1 h of detection time. The LAMP assay was applied to study the dynamics of B. tabaci biotypes both in the field and in greenhouses. It was found that, while temperature may be important for population dynamics of the whitefly in the field, population dynamics in greenhouse conditions may be influenced by the types of insecticide. The newly designed LAMP assay is a simple, rapid and accurate method for identifying the B and Q biotypes. It can be conducted by non-speciali...Continue Reading

References

Apr 18, 2003·Bulletin of Entomological Research·I AbdullahiG Thottappilly
Sep 3, 2003·Journal of Virological Methods·Shiro FukutaYoshiyuki Ishimoto
Mar 10, 2005·Archives of Insect Biochemistry and Physiology·A Rami HorowitzIsaac Ishaaya
Oct 27, 2006·Nature·Rex Dalton
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Dec 10, 2009·The American Journal of Tropical Medicine and Hygiene·Mariangela BonizzoniGuiyun Yan
Jul 7, 2010·Journal of Virological Methods·Cheng-Ping KuanHung-Chang Huang
Feb 12, 2011·Journal of Economic Entomology·Timothy J DennehyXianchun Li

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Citations

Feb 9, 2019·Medical and Veterinary Entomology·C D SchenkelC Silaghi

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Datasets Mentioned

BETA
DQ174533
AY057123
DQ989523
DQ174536
Japan
DQ989532
AJ517768
AJ510081
AY057140
DQ989551

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

PrimerExplorer

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