Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B

Journal of Virological Methods
Masaru IhiraTetsushi Yoshikawa

Abstract

Genotyping of human herpesvirus 6 (HHV-6) is important clinically, particularly for the diagnosis of neurological diseases. The objective of this study was to establish a rapid HHV-6 genotyping method using the loop-mediated isothermal amplification (LAMP) method. An AccI site is located in the target sequence of HHV-6 B, but not in HHV-6 A. LAMP products were digested with the AccI enzyme and then separated by agarose gel electrophoresis to differentiate the digest pattern of the two variants. The fragment patterns were clearly different between HHV-6 A and B. In order to evaluate the reliability of this HHV-6 genotyping method for use in the clinical laboratory, serum samples from 20 patients with either primary HHV-6 infection or viral reactivation were collected and analyzed. HHV-6 DNA was amplified directly from the serum samples and all 20 LAMP products were positive for HHV-6 B.

References

Feb 10, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·C B HallS Dewhurst
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Sep 27, 2005·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·David BoutolleauAgnès Gautheret-Dejean
Mar 23, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Masaru IhiraTetsushi Yoshikawa

❮ Previous
Next ❯

Citations

Dec 24, 2010·Journal of Clinical Microbiology·Qiaoyun LuG S de Hoog
Sep 13, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Zhejun CaiNanping Wu
Sep 8, 2009·Trends in Parasitology·Panagiotis Karanis, Jerry Ongerth
Jun 16, 2018·Journal of Medical Virology·Hiroki MiuraTetsushi Yoshikawa
Jul 2, 2019·The Pediatric Infectious Disease Journal·Fumihiko HattoriTetsushi Yoshikawa
Jan 24, 2020·Journal of the Pediatric Infectious Diseases Society·Hiroki MiuraTetsushi Yoshikawa
Oct 28, 2019·Transplant Infectious Disease : an Official Journal of the Transplantation Society·Hiroki MiuraTetsushi Yoshikawa
May 26, 2018·Transplant Infectious Disease : an Official Journal of the Transplantation Society·Hiroki MiuraTetsushi Yoshikawa
Sep 29, 2020·The Journal of Infectious Diseases·Hiroki MiuraTetsushi Yoshikawa
May 1, 2021·Microorganisms·Misa MiyakeTetsushi Yoshikawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The New England Journal of Medicine
Danielle M ZerrLawrence Corey
Nucleic Acids Research
Tsugunori NotomiT Hase
Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Seiko IwataHiroshi Kimura
Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
David BoutolleauAgnès Gautheret-Dejean
© 2022 Meta ULC. All rights reserved