Abstract
Human papillomavirus (HPV)-related oropharyngeal squamous cell carcinoma (OPSCC) is a growing problem that presents a significant challenge to Otolaryngologist-Head and Neck Surgeons. Knowledge of HPV status yields critical prognostic information, with potential for treatment selection based on tumour HPV status. The current gold standard of diagnosis, PCR, is expensive, demanding and time consuming. Alternatives such as p16 immunohistochemistry are subjective and potentially inaccurate. Loop-mediated isothermal amplification (LAMP) is a rapid, robust and inexpensive molecular diagnostic technique. Our aim was to verify LAMP as a potential bedside diagnostic assay for subtyping of HPV in OPSCC. DNA from 72 formalin-fixed paraffin embedded (FFPE) OPSCC patient samples was tested. PCR and LAMP were then performed to specifically identify HPV 16, 18, 31, 33 and 35. For these high-risk subtypes, LAMP had an overall sensitivity of 99.4% and specificity of 93.2% relative to PCR. LAMP is a promising technology that can accurately diagnose high-risk HPV infection.
References
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Feb 25, 2003·The American Journal of Pathology·Jens P KlussmannPawel G Fuchs
Jun 7, 2003·Journal of Clinical Microbiology·Tomotada IwamotoKozaburo Hayashi
Mar 1, 2005·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Aimee R KreimerSilvia Franceschi
Mar 28, 2007·Journal of Medical Virology·Masanori HagiwaraHidemi Nakagawa
Feb 1, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Anil K ChaturvediMaura L Gillison
Feb 14, 2008·Journal of the National Cancer Institute·Carole FakhryMaura L Gillison
May 3, 2008·Nature Protocols·Norihiro TomitaTsugunori Notomi
May 22, 2008·Journal of Surgical Oncology·Arlene A Forastiere
Oct 2, 2009·International Journal of Cancer. Journal International Du Cancer·Alain C JungBohdan Wasylyk
Jun 10, 2010·The New England Journal of Medicine·K Kian AngMaura L Gillison
Aug 11, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Danny RischinGrant A McArthur
Sep 30, 2010·International Journal of Cancer. Journal International Du Cancer·Maria BlombergSusanne Krüger Kjaer
Feb 15, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M R PosnerK J Cullen
May 17, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Nicolas F SchlechtMichael B Prystowsky
Sep 10, 2011·Journal of Virological Methods·Chitladda SaetiewJureerut Daduang
Oct 5, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Anil K ChaturvediMaura L Gillison
Nov 11, 2011·Cancer Science·Masayuki KabeyaYuichi Ishikawa
Jul 13, 2012·Head and Neck Pathology·Max RobinsonSelvam Thavaraj
Sep 11, 2012·Biosensors & Bioelectronics·Preeda PrakrankamanantPatcharee Jearanaikoon
Oct 9, 2012·Head & Neck·Robert SemrauSimon F Preuss
Oct 2, 2013·Cancer Treatment Reviews·Aru PanwarApar Kishor Ganti
Mar 14, 2014·International Journal of Cancer. Journal International Du Cancer·H MirghaniJ Lacau St Guily
May 3, 2014·Journal of Clinical Microbiology·Manoj NimeshDaman Saluja
Citations
Oct 30, 2016·Journal of Virological Methods·Xuan WangLimin Yang
Mar 15, 2018·British Journal of Biomedical Science·Q ZhongW Liu
Feb 10, 2018·BMC Cancer·Mitchell G RohatenskyGuido van Marle
Feb 9, 2019·Lab on a Chip·Jason E KreutzDaniel T Chiu
Feb 28, 2019·BMC Infectious Diseases·Anargyros SkoulakisEfthymia Petinaki
Feb 4, 2021·Analytical Chemistry·Jiasi WangDaniel T Chiu
Jan 26, 2021·Frontiers in Molecular Biosciences·Lin LinXiaofeng Jiang
Feb 14, 2021·Archives of Oral Biology·Nurul Izzati HamzanSuharni Mohamad
Apr 13, 2021·Journal of Zhejiang University. Science. B·Fatin Hazwani FauziSuharni Mohamad
Feb 3, 2021·Journal of Microbiology and Biotechnology·Donghong ZhangXiulan Ma
Aug 9, 2021·Molecular and Cellular Probes·Dat T Vo, Michael D Story