Loop-mediated isothermal amplification (LAMP) assays for Anopheles funestus group and Anopheles gambiae complex species.

Medical and Veterinary Entomology
T KamberA Mathis

Abstract

Species of the genus Anopheles vary with regard to their vector capacity for Plasmodium spp., the causative agent of malaria, and their accurate identification is often required. Loop-mediated isothermal amplification (LAMP) is a rapid, simple and low-cost method for specific DNA amplification. Primers for LAMP assays specific for the Anopheles funestus group and Anopheles gambiae complex species as well as for the species Anopheles arabiensis, An. funestus, An. gambiae s.s/Anopheles coluzzii (major vectors) and Anopheles rivulorum (minor vector) were designed targeting specific genome or rDNA internal transcribed spacer regions. Reaction conditions (buffer composition, primer concentrations, incubation time) were evaluated and the specificities of the assays confirmed with DNA from non-target Anopheles species. DNA release from the mosquitoes is achieved simply by heating them for 5 min in water. An aliquot of the DNA solutions is transferred to the reaction tube using disposable inoculation loops. The outcome of the LAMP amplifications after 1 h incubation at 65 °C can easily be visualized by a colour change visible to the naked eye. The assays are operable under field conditions requiring only basic equipment (portable heat ...Continue Reading

References

Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·N J BesanskyF H Collins
Oct 1, 1993·The American Journal of Tropical Medicine and Hygiene·J A ScottF H Collins
Feb 1, 1996·Insect Molecular Biology·F H Collins, S M Paskewitz
Sep 13, 2002·The American Journal of Tropical Medicine and Hygiene·L L KoekemoerM Coetzee
Oct 3, 2006·Journal of Biochemical and Biophysical Methods·Hisatoshi KanekoTatsuo Suzutani
Apr 28, 2009·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Yasuyoshi Mori, Tsugunori Notomi
Dec 10, 2009·The American Journal of Tropical Medicine and Hygiene·Mariangela BonizzoniGuiyun Yan
Apr 6, 2012·Parasites & Vectors·Marianne E SinkaSimon I Hay
Dec 3, 2014·Parasites & Vectors·Constentin DiemeLionel Almeras
Apr 9, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·De-Guo WangPeggy M Tomasula
Feb 18, 2017·Molecular Ecology Resources·Patricia L M Lee
Feb 9, 2019·Medical and Veterinary Entomology·C D SchenkelC Silaghi

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