Loop replacements with gut-binding peptides in Cry1Ab domain II enhanced toxicity against the brown planthopper, Nilaparvata lugens (Stål)

Scientific Reports
Ensi ShaoZhipeng Huang

Abstract

Bacillus thuringiensis (Bt) Cry toxins have been used widely in pest managements. However, Cry toxins are not effective against sap-sucking insects (Hemiptera), which limits the application of Bt for pest management. In order to extend the insecticidal spectrum of Bt toxins to the rice brown planthopper (BPH), Nilaparvata lugens, we modified Cry1Ab putative receptor binding domains with selected BPH gut-binding peptides (GBPs). Three surface exposed loops in the domain II of Cry1Ab were replaced with two GBPs (P2S and P1Z) respectively. Bioassay results showed that toxicity of modified toxin L2-P2S increased significantly (~9 folds) against BPH nymphs. In addition, damage of midgut cells was observed from the nymphs fed with L2-P2S. Our results indicate that modifying Cry toxins based on the toxin-gut interactions can broaden the insecticidal spectrum of Bt toxin. This method provides another approach for the development of transgenic crops with novel insecticidal activity against hemipteran insects and insect populations resistant to current Bt transgenic crops.

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Citations

Dec 7, 2018·Protein Science : a Publication of the Protein Society·Xuping JingMeiying Gao
Nov 28, 2017·Applied Microbiology and Biotechnology·Bhupendra S PanwarSarvjeet Kaur
Mar 23, 2018·Scientific Reports·Mary-Carmen Torres-QuinteroAlejandra Bravo
Jun 19, 2021·Frontiers in Microbiology·Lu LiuJin He

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Methods Mentioned

BETA
transgenic
phage display
Protein Assay
electrophoresis
X-ray
PCR
transmission electron microscopy

Software Mentioned

SPSS

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