Loquat (Eriobotrya japonica) leaf extract inhibits the growth of MDA-MB-231 tumors in nude mouse xenografts and invasion of MDA-MB-231 cells

Nutrition Research and Practice
Mi-Kyoung YouHyeon-A Kim


The present study was conducted to examine the inhibitory effect of loquat leaves on MDA-MB-231 cell proliferation and invasion. Female athymic nude mice were given a subcutaneous (s.c.) inoculation of MDA-MB-231 cells and randomly grouped to receive a s.c. injection of either 500 mg/kg ethanol, water extract or vehicle five times a week. Tumor growth, mitotic rate and necrosis were examined. MDA-MB-231 cells were cultured with DMSO or with various concentrations of loquat water or ethanol extract. Proliferation, adhesion, migration, invasion and matrix metalloproteinase (MMP) activity were examined. Tumor growth of xenograft nude mouse was significantly reduced by loquat extracts. The results of mitotic examination revealed that loquat extracts reduced tumor cell division. Both ethanol and water extracts significantly inhibited MDA-MB-231 cell proliferation. The protein expression of ErbB3 was significantly down-regulated by loquat leaf extracts. Loquat leaf extracts increased apoptosis of MDA-MB-231 cells following 24 hour incubation and the ethanol extract was more potent in inducing apoptosis than the water extract. Furthermore, loquat extracts inhibited adhesion, migration and invasion of MDA-MB-231 cells. MMP activity was...Continue Reading


Dec 1, 1995·Journal of Ethnopharmacology·J Liu
May 24, 2000·Chemical & Pharmaceutical Bulletin·Hideyuki ItoT Yoshida
Aug 19, 2000·Cellular and Molecular Life Sciences : CMLS·N JohanssonV M Kähäri
Nov 30, 2000·Breast Cancer Research and Treatment·Q MengS Fan
Feb 7, 2002·Phytochemistry·Shoko TaniguchiTakashi Yoshida
Jul 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Thomas HolbroNancy E Hynes
May 3, 2005·Nature Reviews. Cancer·Nancy E Hynes, Heidi A Lane
Oct 6, 2005·Biological & Pharmaceutical Bulletin·Norihiro BannoHoyoku Nishino
Jun 1, 1990·Planta medica·Z Z LiangC Pizza
Jun 5, 2008·Cancer Letters·Georg F Weber
Feb 12, 2009·Current Opinion in Cell Biology·Nancy E Hynes, Gwen MacDonald
Nov 17, 2009·Breast Cancer Research : BCR·Eva Tomaskovic-CrookJean Paul Thiery
May 5, 2010·Journal of Mammary Gland Biology and Neoplasia·Evette S Radisky, Derek C Radisky
Mar 19, 2013·Biochemical Pharmacology·Muthu K ShanmugamAnupam Bishayee

Methods Mentioned

protein assay

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