Losartan Ameliorates Calcium Oxalate-Induced Elevation of Stone-Related Proteins in Renal Tubular Cells by Inhibiting NADPH Oxidase and Oxidative Stress

Oxidative Medicine and Cellular Longevity
Baolong QinShaogang Wang

Abstract

Calcium oxalate (CaOx) is the most common type of urinary stone. Increase of ROS and NADPH oxidase gives rise to inflammation and injury of renal tubular cells, which promotes CaOx stone formation. Recent studies have revealed that the renin-angiotensin system might play a role in kidney crystallization and ROS production. Here, we investigated the involvement of Ang II/AT1R and losartan in CaOx stone formation. NRK-52E cells were incubated with CaOx crystals, and glyoxylic acid-induced hyperoxaluric rats were treated with losartan. Oxidative stress statuses were evaluated by detection of ROS, oxidative products (8-OHdG and MDA), and antioxidant enzymes (SOD and CAT). Expression of NADPH oxidase subunits (Nox2 and Nox4), NF-κB pathway subunits (p50 and p65), and stone-related proteins such as OPN, CD44, and MCP-1 was determined by Western blotting. The results revealed upregulation of Ang II/AT1R by CaOx treatment. CaOx-induced ROS and stone-related protein upregulation were mediated by the Ang II/AT1R signaling pathway. Losartan ameliorated renal tubular cell expression of stone-related proteins and renal crystallization by inhibiting NADPH oxidase and oxidative stress. We conclude that losartan might be a promising preventive...Continue Reading

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Citations

Dec 5, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Chunsheng WangYingwei Zhang
Sep 24, 2020·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Aleksandra PiotrowskaMarzenna Podhorska-Okolow
Mar 28, 2020·Oxidative Medicine and Cellular Longevity·Kehua JiangFa Sun
May 24, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yihang LiXi Chen
Nov 10, 2020·Journal of Alzheimer's Disease : JAD·Xinquan LiBiao Cai
Apr 16, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Tao DingZhiyong Guo
Feb 14, 2021·Clinical and Translational Science·Junxiang WenJian Li
Aug 2, 2021·Chemico-biological Interactions·Qing-Lin YeDong-Dong Xie
Aug 27, 2021·Antioxidants & Redox Signaling·Yang XunShaogang Wang

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Methods Mentioned

BETA
transfection
Flow
MDA
ELISA
Polymerase Chain Reaction
PCR
electrophoresis
flow cytometry

Software Mentioned

SPSS

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