Loss and resynthesis of a developmentally regulated membrane protein (gp80) during dedifferentiation and redifferentiation in Dictyostelium

Developmental Biology
R E FinneyW F Loomis

Abstract

When developing cultures of Dictyostelium discoideum are disaggregated and resuspended in nutrient medium, they lose the capacity to rapidly reaggregate after 90 min, in a rapid and synchronous step referred to as the "erasure event." They then proceed to lose remaining developmentally acquired functions in a program of dedifferentiation culuminating with the loss of EDTA-resistant cohesion roughly 5 hr later. Immediately following the erasure event, cells can be stimulated to reenter the developmental program even though they still possess a number of developmentally acquired functions. These cells therefore appear to undergo dedifferentiation and redifferentiation simultaneously (D. R. Soll and L. H. Mitchell, 1982, Dev. Biol. 91, 183-190). In this report, we have employed an antiserum made against a developmentally acquired membrane glycoprotein, gp80, to examine whether gp80 is lost during dedifferentiation and whether it is either reutilized or resynthesized during redifferentiation. Results are presented which demonstrate that (1) when 9-hr developing cells are disaggregated and resuspended in nutrient medium, gp80 continues to accumulate for several hours after the erasure event, then is lost at roughly the same time as ...Continue Reading

References

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Citations

Apr 1, 1987·Developmental Biology·R FinneyD R Soll
May 19, 2011·Development, Growth & Differentiation·Emily Roberge-White, Mariko Katoh-Kurasawa
Apr 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mariko KatohGad Shaulsky
Feb 1, 1995·Differentiation; Research in Biological Diversity·N A HopperW F Loomis
Jan 1, 1985·Differentiation; Research in Biological Diversity·S AlexanderD R Soll
Apr 8, 2020·ELife·John Me NicholsJonathan R Chubb

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