Loss of 5-hydroxytryptamine from mammalian circulating labelled platelets

The Journal of Physiology
E E Osim, J H Wyllie

Abstract

1. Platelets were obtained from three species of animal: rats, rabbits and dogs. They were labelled with (111)In oxine to tag individual platelets and with (14)C-labelled 5-hydroxytryptamine (5-HT). Doubly labelled platelets from rabbits and dogs were returned to their donors; in the case of rats the platelets were injected intravenously into other, identical rats. At time intervals from 2 to 64 hr, blood samples were drawn and platelets were collected. (111)In and (14)C were separately counted. In some experiments animals received the 5-HT precursor, 5-hydroxytryptophan (5-HTP) I.P. (for rats and rabbits) or subcutaneously (for dogs) in a dose of 20 mg/kg daily to accelerate synthesis of 5-HT.2. (111)In disappeared in approximately an exponential fashion in all experiments and the rate of disappearance was not affected by treatment with 5-HTP. The half-life for (111)In in four control rats was 18.7 hr and in five rats treated with 5-HTP was 17.8 hr. In rabbits the half-life was 20.4 hr for eight control and 21.2 hr for seven treated with 5-HTP. In the dogs the half-life was 21.0 hr for control and 27.7 hr for experiments with 5-HTP. In control rats, the (14)C behaved like the (111)In. However, in control rabbits the half-life ...Continue Reading

Citations

Jan 1, 1990·Cardiovascular Drugs and Therapy·J I Robertson
Apr 18, 2018·Clinical Pharmacology and Therapeutics·Martine GehinJasper Dingemanse
Feb 23, 2000·Journal of Cardiovascular Pharmacology and Therapeutics·E OsimO Munjeri
Apr 10, 2021·Physiological Reports·Meghan K ConnellyLaura Hernandez

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