Loss of a single N-linked glycan from the hemagglutinin of influenza virus is associated with resistance to collectins and increased virulence in mice.

Respiratory Research
Patrick C ReadingAndrew G Brooks

Abstract

Glycosylation on the globular head of the hemagglutinin (HA) protein of influenza virus acts as an important target for recognition and destruction of virus by innate immune proteins of the collectin family. This, in turn, modulates the virulence of different viruses for mice. The role of particular oligosaccharide attachments on the HA in determining sensitivity to collectins has yet to be fully elucidated. When comparing the virulence of H3N2 subtype viruses for mice we found that viruses isolated after 1980 were highly glycosylated and induced mild disease in mice. During these studies, we were surprised to find a small plaque variant of strain A/Beijing/353/89 (Beij/89) emerged following infection of mice and grew to high titres in mouse lung. In the current study we have characterized the properties of this small plaque mutant both in vitro and in vivo. Small plaque mutants were recovered following plaquing of lung homogenates from mice infected with influenza virus seed Beij/89. Compared to wild-type virus, small plaque mutants showed increased virulence in mice yet did not differ in their ability to infect or replicate in airway epithelial cells in vitro. Instead, small plaque variants were markedly resistant to neutrali...Continue Reading

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Citations

Jun 12, 2012·The Journal of Biological Chemistry·Martin van EijkBarbara A Seaton
Jul 27, 2011·BMC Evolutionary Biology·Trevor BedfordMercedes Pascual
Nov 27, 2015·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Karen LittleAeron C Hurt
Apr 2, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jonathan RunstadlerMandy Keogh
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Jan 31, 2015·Biochemical and Biophysical Research Communications·Jinlei GuoJianfang Zhou
Jun 8, 2018·Glycobiology·Victoria OrtegaHector C Aguilar
Nov 30, 2011·Journal of Leukocyte Biology·Sarah L LondriganPatrick C Reading
Aug 8, 2012·Yonsei Medical Journal·Jin Il Kim, Man-Seong Park
Jul 6, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kevan L HartshornErika C Crouch
Jul 20, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michelle D TatePatrick C Reading
Dec 12, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julia HütterBernd Lepenies
Feb 1, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emma R JobPatrick C Reading
Dec 7, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emma R JobPatrick C Reading
Dec 9, 2020·Antimicrobial Agents and Chemotherapy·Evelien VanderlindenDominique Schols

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Datasets Mentioned

BETA
L76036

Methods Mentioned

BETA
glycosylation
lavage
bronchoalveolar lavage
FCS
PCR

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