Loss of acidification of anterior prostate fluids in Atp12a-null mutant mice indicates that nongastric H-K-ATPase functions as proton pump in vivo

American Journal of Physiology. Cell Physiology
Nikolay B PestovNikolai N Modyanov

Abstract

The physiological functions of nongastric (colonic) H-K-ATPase (gene symbol Atp12a), unlike those of Na-K-ATPase and gastric H-K-ATPase, are poorly understood. It has been suggested that it pumps Na+ more efficiently than H+; however, so far, there is no direct evidence that it pumps H+ in vivo. Previously, we found that the nongastric H-K-ATPase alpha-subunit is expressed in apical membranes of rodent anterior prostate epithelium, in a complex with the Na-K-ATPase beta1-subunit. Here we report the effects of Atp12a gene ablation on polarization of the beta1-subunit and secretory function of the anterior prostate. In nongastric H-K-ATPase-deficient prostate, the Na-K-ATPase alpha-subunit resided exclusively in basolateral membranes; however, the beta1-subunit disappeared from apical membranes, demonstrating that beta1 is an authentic subunit of nongastric H-K-ATPase in vivo and that apical localization of beta1 in the prostate is completely dependent on its association with the nongastric H-K-ATPase alpha-subunit. A remarkable reduction in acidification of anterior prostate fluids was observed: pH 6.38 +/- 0.14 for wild-type mice and 6.96 +/- 0.10 for homozygous mutants. These results show that nongastric H-K-ATPase is required...Continue Reading

References

Apr 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K X Gao, J D Godkin
Apr 1, 1990·The American Journal of Physiology·T WatanabeY Suzuki
Jan 1, 1984·Molecular and Cellular Biochemistry·H G Williams-Ashman
Oct 1, 1995·The American Journal of Physiology·N N ModyanovK Geering
Oct 1, 1995·The Journal of Clinical Investigation·J LeeH J Binder
Mar 29, 1996·The Journal of Biological Chemistry·M CougnonF Jaisser
Oct 1, 1996·The Journal of Clinical Investigation·J WangA A McDonough
Sep 1, 1996·The American Journal of Physiology·A V GrishinM J Caplan
Jun 13, 1997·The Journal of Biological Chemistry·N HaradaM Morikawa
Mar 21, 1998·The Journal of Clinical Investigation·P MenetonG E Shull
Mar 5, 1998·The American Journal of Physiology·V M RajendranH J Binder
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M CougnonF Jaisser
Jul 14, 1998·Nature Genetics·P J SchultheisG E Shull
Sep 8, 1998·The American Journal of Physiology·J CodinaT D DuBose
Oct 17, 1998·The Journal of Biological Chemistry·A V Grishin, M J Caplan
Jun 11, 1999·The American Journal of Physiology·R B Silver, M Soleimani
Aug 13, 1999·The American Journal of Physiology·M CougnonG Planelles
Sep 1, 1999·Kidney International·G Fejes-TóthH Velázquez
Jan 15, 2000·The Journal of Biological Chemistry·G CrambertK Geering
Jan 25, 2000·American Journal of Physiology. Cell Physiology·P SanganH J Binder
Feb 9, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·G E ShullP J Schultheis
Apr 25, 2000·The Journal of Biological Chemistry·V M RajendranH J Binder
Sep 1, 2000·American Journal of Physiology. Renal Physiology·J ReinhardtM J Caplan
Jul 18, 2001·American Journal of Physiology. Renal Physiology·G Fejes-Tóth, A Náray-Fejes-Tóth
Jul 18, 2001·American Journal of Physiology. Renal Physiology·J W VerlanderC S Wingo
Sep 13, 2001·American Journal of Physiology. Renal Physiology·J A KrautG Sachs
Nov 14, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·Z SpicerG E Shull
Jan 5, 2002·Journal of Bioenergetics and Biomembranes·K Geering
Mar 7, 2002·American Journal of Physiology. Cell Physiology·Nikolay B PestovNikolai N Modyanov

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Citations

Feb 27, 2013·Pflügers Archiv : European journal of physiology·Amel SalhiGilles Crambert
Oct 28, 2010·The Journal of Biological Chemistry·Ivana NovakSusanne E Hede
Jul 31, 2009·American Journal of Physiology. Renal Physiology·Michelle L GumzCharles S Wingo
Mar 8, 2008·Pflügers Archiv : European journal of physiology·Jan Joep H H M De PontJan B Koenderink
May 16, 2009·Drug Discovery Today·James M MullinJames J Thornton
Feb 18, 2014·British Journal of Pharmacology·Stephen P H AlexanderUNKNOWN CGTP Collaborators
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Doris StreifMarkus Ritter
Jul 10, 2010·Current Opinion in Nephrology and Hypertension·Megan M GreenleeCharles S Wingo
Jul 11, 2006·American Journal of Physiology. Cell Physiology·Toshiaki ShibataYoshihisa Kurachi
Jun 13, 2019·American Journal of Physiology. Renal Physiology·Lydie ChevalAlain Doucet
Jan 17, 2014·American Journal of Physiology. Renal Physiology·Gilles Crambert
Mar 31, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Amel SalhiGilles Crambert
Oct 20, 2018·JCI Insight·Paolo ScudieriLuis Jv Galietta
Dec 29, 2020·International Journal of General Medicine·Zhe ZhangShui-Hong Zhou
Sep 12, 2007·Journal of Proteome Research·Mark DonowitzXuhang Li

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