PMID: 6166715May 1, 1981Paper

Loss of an individual idiotype on chemical modification. A strategy for assigning idiotypic determinants

The Journal of Experimental Medicine
J DickermanB Friedenson

Abstract

Two dextran-binding myeloma proteins, J558 and Hdex 24, which possess the same individual idiotype (IdI) were diazotized to low levels (1-3.3 groups per subunit) with 1-[14C]-p-aminobenzoate. Both proteins lost the IdI idiotype under these conditions with most of the label incorporated on the heavy chains of each protein. When the diazotization ws carried out in the presence of the hapten 1-O-methyl-alpha-D-glucopyranoside the loss of idiotypic reactivity could be prevented for J558 but not for Hdex 24. Under these conditions most of the label was incorporated on the light chains of J558, but on the heavy chains of Hdex 24. For J558, these results show that a major determinant of the individual idiotype is within the hypervariable positions of the heavy chain. For Hdex 24 the determinant being modified is on the heavy chain but not involved in hapten binding. These results are consistent with previous work showing that J558 and Hdex 24 differ in amino acid sequence in the D and the J segments of the heavy chain and offer an alternative and complementary strategy for assigning idiotypic determinants.

References

Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·C A JanewayH N Eisen
Jun 1, 1977·European Journal of Immunology·S T JuA Nisonoff
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M KehryL E Hood
Apr 1, 1973·The Journal of Experimental Medicine·L L Pawlak, A Nisonoff
Apr 1, 1974·The Journal of Experimental Medicine·R LiebermanS Rudikoff
Dec 1, 1974·The Journal of Experimental Medicine·T Imanishi, O Mäkelä
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·I M Cesari, M Weigert
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N M Gough, O Bernard
Jul 1, 1964·Biochemistry·H N EISEN, G W SISKIND

❮ Previous
Next ❯

Citations

Apr 6, 1988·Journal of Immunological Methods·F J StevensN S Greenspan
Jan 1, 1990·International Reviews of Immunology·R Attanasio, R C Kennedy
Jun 17, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tamer I MahmoudJohn F Kearney

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.