Loss of carbamoyl phosphate synthetase I in small-intestinal adenocarcinoma

American Journal of Clinical Pathology
Diana M CardonaChen Liu

Abstract

Carbamoyl phosphate synthetase I (CPS1), normally found in hepatocytes and small-intestine (SI) enterocytes, is the antigen of Hep Par 1 antibody. Expression of CPS1 in invasive SI adenocarcinoma seems to be lost. We retrospectively collected 36 total specimens, which included 31 SI adenomas and 21 adenocarcinomas. We used 34 cases of duodenitis as a control group. Immunohistochemical and Western blot analyses were performed to determine CPS1 expression. The normal SI mucosa, all 34 cases of duodenitis, and all 29 adenomas with low-grade dysplasia demonstrated diffuse Hep Par 1 expression. Of the 21 invasive adenocarcinomas, 15 lost antigen expression (71%). These data are statistically significant (P < .05). Western blot analysis confirmed the immunohistochemical findings, with strong CPS1 expression within the normal mucosa and adenoma and complete loss in the invasive tumor. The differential expression of Hep Par 1 in dysplastic vs malignant tumors of the SI may be diagnostically useful in difficult cases.

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Citations

Dec 20, 2011·American Journal of Clinical Pathology·Jennifer A JeungDiana M Cardona
Jun 2, 2015·Archives of Pathology & Laboratory Medicine·Stephen LaganaHelen Remotti
Aug 28, 2012·Metabolism: Clinical and Experimental·Maria M AdevaCristóbal Donapetry
Feb 12, 2017·Applied Immunohistochemistry & Molecular Morphology : AIMM·Sejal S Shah, Vishal S Chandan
Jun 20, 2012·Applied Immunohistochemistry & Molecular Morphology : AIMM·Gabriel Moises GroismanAlona Meir
Sep 9, 2018·Nature Reviews. Cancer·Rom KeshetAyelet Erez
Jun 30, 2019·International Journal of Molecular Sciences·Petr DanielJan Kovář
Dec 16, 2020·Molecular Genetics and Metabolism·Matthew Nitzahn, Gerald S Lipshutz
Jun 27, 2014·Journal of Proteome Research·Masato TaokaTadashi Kondo

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