Loss of communication in ovarian cancer

American Journal of Obstetrics and Gynecology
Chris R TolerCicek Gercel-Taylor

Abstract

This study was undertaken to investigate the expression of connexins in ovarian cancer cell lines and assess correlation with chemosensitivity. Eight cell lines were used in this study. Cytotoxicity assays with carboplatin and paclitaxel were performed. Reverse transcription-polymerase chain reaction (RT-PCR) was used to analyze the expression of gap junction proteins. Western blotting and immunohistochemical staining of selective gap junction proteins (connexin 26 and 43) were performed. All 11 connexins were detected with RT-PCR profiling in ovarian cancer cells. Further experiments were performed to analyze protein expression with connexins 26 and 43. Levels of connexin proteins did not correlate with gene expression obtained with RT-PCR. Results obtained with immunoblotting were confirmed by immunohistochemistry. Carboplatin and paclitaxel sensitivities were determined and connexin 43 expression was associated with sensitivity to paclitaxel. Expression of connexins is regulated at the translational level in ovarian cancer. Sensitivity to paclitaxel maybe associated with connexin 43.

References

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Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S W LeeR Sager
May 20, 2000·American Journal of Obstetrics and Gynecology·S UmhauerJ Fanning
Jul 20, 2002·Experimental and Molecular Pathology·Martha J FernstromRandall J Ruch

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Citations

Nov 2, 2014·BMC Bioinformatics·Elisabetta FersiniFrancesco Archetti
Dec 29, 2007·Molecular and Cellular Endocrinology·Eran GershonNava Dekel
May 29, 2008·Journal of Cellular and Molecular Medicine·H-X WangG M Kidder
Jan 6, 2021·International Journal of Molecular Sciences·Mauricio Serrano-RubiArturo Ponce

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