Loss of decorin from the surface zone of articular cartilage in a chick model of osteoarthritis

Acta histochemica
P G MasséD S Howell

Abstract

The objective of this study was to immunolocalize decorin and to assess changes as a result of pyridoxine (PN) deficiency in chick articular cartilage from femoral condyles. After maintenance on a normal diet for the first two weeks after hatching, 15 broiler chickens were deprived of this vitamin for 6 weeks. It was previously shown that the ankle joints of PN-deficient animals are swollen with effusions. They also present an abnormal gait, enlarged bony margins, and fissuring of the articular cartilages. Milder changes (no fissures) were also shown in the knee joints. Data from a previous study were suggestive that sulfated glycosaminoglycans are lost from the knee cartilage surface into synovial fluid. The current study was focused on the small proteoglycan, decorin, which coats the surface of collagen fibrils and may regulate their morphology. To examine decorin in normal and PN-deficient articular cartilage, a monoclonal antibody to an epitope on the protein core of decorin was used for immunohistochemical staining of tissue sections and for Western Blot analysis of cartilage extracts. Reduction of staining with the antibody was demonstrated in the tangential surface zone of PN-deficient cartilage, and Western Blot analysi...Continue Reading

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Apr 25, 2000·Archives of Oral Biology·M Del SantoR J Hinton
Jun 1, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Marie-Pierre Hellio Le GraverandDavid A Hart
Dec 2, 2000·Canadian Journal of Physiology and Pharmacology·P G MasséD S Howell

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