Loss of Elongation-Like Factor 1 Spontaneously Induces Diverse, RNase H-Related Suppressor Mutations in Schizosaccharomyces pombe

Genetics
Bahjat F MarayatiKe Zhang

Abstract

A healthy individual may carry a detrimental genetic trait that is masked by another genetic mutation. Such suppressive genetic interactions, in which a mutant allele either partially or completely restores the fitness defect of a particular mutant, tend to occur between genes that have a confined functional connection. Here we investigate a self-recovery phenotype in Schizosaccharomyces pombe, mediated by suppressive genetic interactions that can be amplified during cell culture. Cells without Elf1, an AAA+ family ATPase, have severe growth defects initially, but quickly recover growth rates near to those of wild-type strains by acquiring suppressor mutations. elf1Δ cells accumulate RNAs within the nucleus and display effects of genome instability such as sensitivity to DNA damage, increased incidence of lagging chromosomes, and mini-chromosome loss. Notably, the rate of phenotypic recovery was further enhanced in elf1Δ cells when RNase H activities were abolished and significantly reduced upon overexpression of RNase H1, suggesting that loss of Elf1-related genome instability can be resolved by RNase H activities, likely through eliminating the potentially mutagenic DNA-RNA hybrids caused by RNA nuclear accumulation. Using wh...Continue Reading

References

Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·U H MortensenR Rothstein
Dec 28, 1999·Methods : a Companion to Methods in Enzymology·M D Manson
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·S S EaglestoneM F Tuite
Sep 5, 2001·Nature Reviews. Genetics·S L Forsburg
Apr 25, 2002·Nature Reviews. Genetics·Daniel St Johnston
May 4, 2002·Nature Reviews. Genetics·Erik M Jorgensen, Susan E Mango
Dec 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Bjorn Rydberg, John Game
Sep 2, 2005·Molecular and Cellular Biology·Fekret OsmanMatthew C Whitby
Sep 2, 2006·Cardiovascular Revascularization Medicine : Including Molecular Interventions·Jaco H HoutgraafWim J van der Giessen
Apr 24, 2007·Nature Structural & Molecular Biology·Estelle NicolasShiv I S Grewal
Feb 12, 2008·Current Protocols in Molecular Biology·Susan L Forsburg
Mar 18, 2008·Nature Structural & Molecular Biology·Ke ZhangShiv I S Grewal
Feb 21, 2009·The FEBS Journal·Susana M Cerritelli, Robert J Crouch
Apr 11, 2009·Nature·Michael R StrattonP Andrew Futreal
Jul 1, 2009·Methods in Molecular Biology·Douglas D Luche, Susan L Forsburg
Dec 18, 2009·Nature·Erin D PleasanceMichael R Stratton
Apr 24, 2010·Science·Robin D DowellCharles Boone
Aug 24, 2010·Nature Chemical Biology·Stephanie A Nick McElhinnyThomas A Kunkel
Oct 23, 2010·Molecular Cell·Alberto Ciccia, Stephen J Elledge
Apr 2, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yuji InoueMasasuke Yoshida
Sep 6, 2011·Nature Structural & Molecular Biology·Francisca E Reyes-TurcuShiv I S Grewal
Nov 18, 2011·Molecular Biology International·Kenji Fukui, Seiki Kuramitsu
May 1, 2012·Molecular Cell·Andrés Aguilera, Tatiana García-Muse
Oct 17, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Benjamin H Lok, Simon N Powell
Oct 30, 2012·The EMBO Journal·Leila Touat-TodeschiniAndré Verdel
Apr 20, 2013·Chemical Reviews·Hélène GaillardAndrés Aguilera
May 24, 2013·Open Biology·Jacqueline HaylesPaul Nurse
Aug 6, 2013·Annual Review of Genetics·Andrés Aguilera, Tatiana García-Muse
Jul 6, 2014·Genes & Development·Konstantina Skourti-Stathaki, Nicholas J Proudfoot
Sep 5, 2014·Nature·Havva KeskinFrancesca Storici
May 6, 2015·Current Opinion in Cell Biology·Lorenzo Costantino, Douglas Koshland
Jun 6, 2015·Trends in Cell Biology·Julie Sollier, Karlene A Cimprich
Oct 28, 2015·Nature Reviews. Genetics·Andrew R HarperEric J Topol

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA471808

Methods Mentioned

BETA
dissection
PCR
confocal microscopy

Software Mentioned

Gen5
JMP
SPSS
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved