Loss-of-function of ASPARTIC PEPTIDASE NODULE-INDUCED 1 (APN1) in Lotus japonicus restricts efficient nitrogen-fixing symbiosis with specific Mesorhizobium loti strains

The Plant Journal : for Cell and Molecular Biology
Hiroko Yamaya-ItoYosuke Umehara

Abstract

The nitrogen-fixing symbiosis of legumes and Rhizobium bacteria is established by complex interactions between the two symbiotic partners. Legume Fix- mutants form apparently normal nodules with endosymbiotic rhizobia but fail to induce rhizobial nitrogen fixation. These mutants are useful for identifying the legume genes involved in the interactions essential for symbiotic nitrogen fixation. We describe here a Fix- mutant of Lotus japonicus, apn1, which showed a very specific symbiotic phenotype. It formed ineffective nodules when inoculated with the Mesorhizobium loti strain TONO. In these nodules, infected cells disintegrated and successively became necrotic, indicating premature senescence typical of Fix- mutants. However, it formed effective nodules when inoculated with the M. loti strain MAFF303099. Among nine different M. loti strains tested, four formed ineffective nodules and five formed effective nodules on apn1 roots. The identified causal gene, ASPARTIC PEPTIDASE NODULE-INDUCED 1 (LjAPN1), encodes a nepenthesin-type aspartic peptidase. The well characterized Arabidopsis aspartic peptidase CDR1 could complement the strain-specific Fix- phenotype of apn1. LjAPN1 is a typical late nodulin; its gene expression was exclu...Continue Reading

References

Dec 1, 1971·The Biochemical Journal·W J Broughton, M J Dilworth
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·J T SullivanC W Ronson
Aug 1, 1996·Applied and Environmental Microbiology·J T SullivanC W Ronson
May 20, 2000·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·E AsamizuS Tabata
Sep 7, 2000·Molecular Plant-microbe Interactions : MPMI·E FlemetakisP Katinakis
Feb 24, 2001·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T KanekoS Tabata
Mar 7, 2001·Cellular and Molecular Life Sciences : CMLS·D A DayL Rosendahl
Feb 15, 2002·Molecular Plant-microbe Interactions : MPMI·Masayoshi KawaguchiShoichiro Akao
Feb 21, 2002·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·M HayashiK Harada
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Michael K. Udvardi, David A. Day
May 22, 2004·European Journal of Biochemistry·Isaura Simões, Carlos Faro
Mar 7, 2008·Molecular Plant-microbe Interactions : MPMI·Takaki MaekawaMakoto Hayashi
Jul 29, 2008·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Yoshikazu ShimodaShusei Sato
Feb 28, 2009·Molecular Plant-microbe Interactions : MPMI·Shimpei MagoriMasayoshi Kawaguchi
Nov 6, 2009·Molecular Plant-microbe Interactions : MPMI·Bishun Deo PrasadBharat B Chattoo
Feb 27, 2010·Science·Willem Van de VeldePeter Mergaert
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shengming YangHongyan Zhu
May 6, 2011·Molecular Plant-microbe Interactions : MPMI·Jeremy D Murray
Oct 12, 2011·BMC Plant Biology·Juan Elías OlivaresFederico Sánchez
Oct 25, 2011·Cell Host & Microbe·Guilhem J Desbrosses, Jens Stougaard
Nov 9, 2012·Breeding Science·Masaki HayashiYosuke Umehara
Dec 4, 2012·Applied and Environmental Microbiology·Takahiro TsukuiKiwamu Minamisawa
Mar 5, 2013·Annual Review of Plant Biology·Michael Udvardi, Philip S Poole
Sep 13, 2013·Annual Review of Microbiology·Eva KondorosiAttila Kereszt
Jul 26, 2014·Plant & Cell Physiology·Masaki HayashiYosuke Umehara

❮ Previous
Next ❯

Citations

Dec 16, 2017·The Plant Journal : for Cell and Molecular Biology·Sheila McCormick
Sep 27, 2018·Molecular Plant-microbe Interactions : MPMI·Fathi BerrabahBenjamin Gourion
Sep 12, 2018·Canadian Journal of Microbiology·George C diCenzoAlessio Mengoni
Feb 5, 2019·Journal of Experimental Botany·Anna TornkvistPanagiotis N Moschou
Oct 15, 2019·Cellular Microbiology·Claire BenezechBenjamin Gourion
Jan 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yoshikazu ShimodaMakoto Hayashi
May 23, 2019·Physiologia Plantarum·Akito ImaiNorio Suganuma
Jan 18, 2018·Molecular Plant-microbe Interactions : MPMI·Benjamin Gourion, Benoit Alunni
Dec 5, 2019·Microbial Biotechnology·Kristina Lindström, Seyed Abdollah Mousavi
Sep 28, 2018·Frontiers in Microbiology·María J LoriteMilagros León-Barrios
Feb 12, 2021·Journal of Plant Research·Toshio AokiTomoyoshi Akashi
May 22, 2021·Environmental Microbiology Reports·Lucas P P BragaLaurent Philippot

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.