Loss of function of Oryza sativa Argonaute 18 induces male sterility and reduction in phased small RNAs

Plant Reproduction
Soumita DasP V Shivaprasad

Abstract

In this manuscript, we show that Oryza sativa indica Argonaute protein AGO18 is required for male gametophyte development likely to through a small RNA-mediated mechanism. Monocots have evolved unique gene silencing pathways due to the presence of unique members of Dicer-like and Argonaute (AGO) family members. Among the monocot AGO homologs, AGO18 occupies a unique position. Previous reports have implicated this protein in viral resistance as well as in gametogenesis, likely through its competition with AGO1 clade members for micro(mi)RNAs and other small (s)RNAs. Although expression of rice AGO18 in specific stages of male gametogenesis has been documented, its major functions in plant development remain poorly understood. Here, we show that Oryza sativa indica AGO18 is involved in male gametophyte development. Knockdown (KD) of AGO18 in transgenic rice lines resulted in stunted plants that are male sterile, whereas their carpels were functional. Transcriptome analysis revealed downregulation of several pollen development-associated genes in KD lines. sRNA sequencing in vegetative and reproductive tissues of KD lines indicated reduction of miRNAs and phased secondary sRNAs implicated in male gametophyte development. Our resul...Continue Reading

References

May 10, 2002·The Plant Journal : for Cell and Molecular Biology·Asuka NishimuraMakoto Matsuoka
Jul 9, 2002·Genes & Development·Brenda J ReinhartDavid P Bartel
Aug 31, 2002·Cell·Matthew W RhoadesDavid P Bartel
May 6, 2004·The Plant Journal : for Cell and Molecular Biology·Rubén Darío García-PérezAnna Depicker
Sep 17, 2004·Nature·David Baulcombe
Jan 22, 2005·Plant & Cell Physiology·Jun-Ichi ItohYasuo Nagato
Sep 6, 2005·The Plant Cell·Ki-Hong JungGynheung An
Mar 15, 2006·The Plant Cell·Rebecca SchwabDetlef Weigel
Apr 28, 2006·FEBS Letters·Rogerio MargisPeter M Waterhouse
Jun 24, 2006·The Plant Journal : for Cell and Molecular Biology·Hiroyuki TsujiMakoto Matsuoka
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xiaoyu ZhangSteven E Jacobsen
Aug 22, 2007·Current Opinion in Plant Biology·Matthew R Willmann, R Scott Poethig
Sep 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi NagasakiYutaka Sato
Feb 6, 2008·The Plant Journal : for Cell and Molecular Biology·Xinai ZhaoJohn Bennett
Mar 20, 2008·PloS One·Norman WarthmannPhilippe Hervé
May 30, 2008·Trends in Plant Science·Hervé Vaucheret
Aug 2, 2008·Genes & Genetic Systems·Tomoaki FujiokaMasao Watanabe
Aug 21, 2008·Bioinformatics·Simon MoxonVincent Moulton
Jul 9, 2009·Genome Research·Cameron JohnsonLewis H Bowman
Nov 12, 2009·The Plant Cell·Liang WuYijun Qi
May 25, 2010·Nature Genetics·Kotaro MiuraMotoyuki Ashikari
May 25, 2010·Nature Genetics·Yongqing JiaoJiayang Li
Dec 25, 2010·The Plant Cell·Allison Mallory, Hervé Vaucheret
Mar 2, 2011·Journal of Experimental Botany·Filipe BorgesJörg D Becker
Oct 7, 2011·The Plant Journal : for Cell and Molecular Biology·Xianwei SongXiaofeng Cao
Mar 13, 2012·The Plant Cell·Padubidri V ShivaprasadDavid C Baulcombe
Oct 3, 2012·Journal of Integrative Plant Biology·Jing-Xin Guo, Yao-Guang Liu

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Citations

Aug 25, 2020·Journal of Experimental Botany·Guilherme Silva-MartinsPeter Moffett
Sep 24, 2020·Plant Molecular Biology·Kannan PachamuthuPadubidri V Shivaprasad
Jan 6, 2021·Nature Plants·Yang-Seok LeeJose Gutierrez-Marcos
Mar 3, 2021·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Yunfei LiWeiwei Jin
Jun 3, 2021·Annual Review of Phytopathology·Yi XuXueping Zhou

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Datasets Mentioned

BETA
GSE107904
GSM2883136
GSM2883118
GSM2883135

Methods Mentioned

BETA
transgenic
PCR
RNA-seq
chip
immunoprecipitation

Software Mentioned

Web MicroRNA Designer
UEA small RNA Workbench
ShortStack
miRProf
WMD3

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