Loss of function of vascular smooth muscle cells by nitric oxide-dependent and -independent interactions with tumorigenic cells

International Journal of Cancer. Journal International Du Cancer
Guillaume MorissetteF Marceau

Abstract

Little is known about the interaction of tumor cells with host vascular smooth muscle cells. In reconstitution experiments, tumorigenic cell lines (including the rat hepatocarcinoma Morris 7777 and human melanoma M-21) were cultured for 17 hr in the presence of rat aortic rings, subsequently evaluated in contractility assays (response to phenylephrine and KCl). An agonist-independent loss of contractility was observed in rings pre-incubated with either tumorigenic cell lines or their conditioned medium (CM). The depressing effect of Morris cells depends largely on the expression of inducible nitric oxide synthase (iNOS) in smooth muscle cells and was reversed by an inhibitor of this enzyme; iNOS immunoreactivity was verified in some muscular vessels at the periphery of tumors formed by the Morris cell line in rats. The M-21 melanoma produces cytotoxicity in rat aortic rings (presence of single stranded DNA, cleavage of PARP, in differentiated smooth muscle only), accounting for the irreversible loss of contractility. The cytotoxicity produced by M-21 CM is not dependent on NO. Gel filtration of CM suggests that both the iNOS- and cytotoxicity-inducing substances from Morris hepatoma cells or M-21 cells, respectively, are mainly...Continue Reading

References

Aug 1, 1992·The American Journal of Physiology·F A LaiG Meissner
Nov 1, 1991·Japanese Journal of Cancer Research : Gann·K HoriQ H Zhang
Jan 1, 1997·Cancer Letters·S MurrayM R Albertini
Jun 1, 1997·Virchows Archiv : an International Journal of Pathology·N Wernert
Jan 1, 1997·British Journal of Cancer·M J DworkinT G Allen-Mersh
Jan 23, 1999·The Journal of Clinical Investigation·D C Darland, P A D'Amore
Jul 7, 2001·The Journal of Biological Chemistry·G A McMahonD A Lawrence
Aug 30, 2002·Biochemical and Biophysical Research Communications·Wei-Gang TongThomas E Adrian
Dec 20, 2002·International Immunopharmacology·François MarceauAlbert Adam
Jan 1, 2003·The American Journal of Pathology·Michael S GeeWilliam M F Lee

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Citations

Oct 2, 2009·Canadian Journal of Physiology and Pharmacology·Catherine BrousseauEric Petitclerc
Sep 14, 2010·International Immunopharmacology·François MarceauJohanne Bouthillier
Sep 11, 2013·International Immunopharmacology·Lajos GeraFrançois Marceau
Jan 18, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Jean-Philippe FortinFrançois Marceau
Dec 22, 2005·The Journal of Pharmacology and Experimental Therapeutics·Lajos GeraFrançois Marceau
Oct 14, 2008·Experimental Biology and Medicine·Eduardo FerreroAngel Luis García-Villalon

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