Loss of glucose transporter-2 precedes insulin loss in the nonobese diabetic and the low-dose streptozotocin mouse models: a comparative immunohistochemical study by light and confocal microscopy

General and Comparative Endocrinology
S ReddyJ M Ross

Abstract

Glucose transporter-2 (glut2) is underexpressed in beta cells of several rodent models of non-insulin-dependent diabetes mellitus (NIDDM). This may also be true for rodent models of insulin-dependent diabetes mellitus (IDDM). The present study examines two murine models of autoimmune IDDM, the nonobese diabetic (NOD) and the low-dose streptozotocin (stz) murine models for changes in the expression of glut2 by double-label light and confocal microscopy during various stages of the disease. The spatial distribution of glut2 cells was also examined in relation to insulin immunoreactive cells and the islet inflammatory cells during these stages. In both the female NOD mouse and the female Swiss mouse without stz treatment, glut2 colocalized with insulin in virtually all the beta cells. In the NOD mouse, islets with moderate to advanced insulitis showed either an absence or considerably reduce expression of glut2 in insulin-containing beta cells. Cells with reduced glut2 expression were usually located adjacent to the region of insulitis. At onset of diabetes, glut2 immunolabeling was reduced despite the preservation of weak insulin immunoreactivity. In Swiss mice treated repeatedly with stz, glut2 labeling began to decline in selec...Continue Reading

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Citations

Apr 2, 2009·Biochemical and Biophysical Research Communications·Suwattanee KooptiwutPa-thai Yenchitsomanus
Jul 31, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Beatriz López-EscobarPatricia Ybot-González
Jun 13, 2006·Arquivos brasileiros de endocrinologia e metabologia·Ubiratan Fabres MachadoPatrícia M Seraphim
Aug 4, 2004·Cell Biochemistry and Biophysics·Gladys Teitelman

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