Loss of Gαq impairs regulatory B-cell function

Arthritis Research & Therapy
Yan HeGuixiu Shi

Abstract

Recent studies have shown a crucial role of Gαq in immune regulation, but how Gαq modulates regulatory B-cell (Breg) function is still unclear. We address this here. CD19+IL-10+ Bregs of wild-type (WT) and Gnaq-/- mice were analyzed by flow cytometry after stimulation by lipopolysaccharide. The WT and Gnaq-/- Bregs were isolated and cocultured with WT CD4+CD25- T cells in the presence of T-activator, and the proliferation of T cells and differentiation of regulatory T cells (Tregs) were analyzed by flow cytometry. We used inhibitors of PI3 kinase (PI3K), extracellular regulated protein kinases 1/2 (Erk1/2), and p38 mitogen-activated protein kinase (p38 MAPK) to detect the pathways involved in the regulation of Gαq on Breg differentiation, which were confirmed by western blot analysis. Furthermore, the expression level of Gαq was assessed by quantitative real-time PCR in peripheral blood mononuclear cells (PBMCs) from healthy controls and rheumatoid arthritis patients. The frequency of CD19+CD24hiCD38hi B cells in PBMCs was detected by flow cytometry, and the association of the Gαq mRNA expression level and the frequency of CD19+CD24hiCD38hi B cells was analyzed by Spearman test. The differentiation of CD19+IL-10+ Bregs was inhi...Continue Reading

References

Oct 11, 1974·Nature·S I KatzJ L Turk
Dec 1, 1996·The Journal of Experimental Medicine·S D WolfC A Janeway
Sep 20, 1997·Nature·S OffermannsM I Simon
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·S OffermannsM I Simon
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael T BorchersJames J Lee
Jun 1, 2002·Science·Susana R NevesRavi Iyengar
Sep 24, 2002·Nature Immunology·Simon FillatreauStephen M Anderton
Mar 4, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Michael T BorchersJ J Lee
Feb 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Bo WeiJonathan Braun
Sep 27, 2005·Cellular Signalling·Katherine B Hubbard, John R Hepler
Sep 27, 2005·Physiological Reviews·Nina Wettschureck, Stefan Offermanns
Apr 26, 2007·Journal of Leukocyte Biology·Jane LattinMatthew J Sweet
Mar 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vicky LampropoulouSimon Fillatreau
Jun 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Koichi YanabaThomas F Tedder
Dec 17, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roxana Roohi AhangaraniJean-Marie Saint-Remy
Feb 16, 2010·Nature Reviews. Immunology·Margarida Saraiva, Anne O'Garra
Mar 13, 2010·Nature Reviews. Immunology·Frances E Lund, Troy D Randall
Jul 14, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takashi MatsushitaThomas F Tedder
Dec 15, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ayca SayiAnne Müller
Mar 4, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vinod S RamgolamSilva Markovic-Plese
Apr 6, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Natalie A CarterClaudia Mauri
Jan 10, 2012·Annual Review of Immunology·Claudia Mauri, Anneleen Bosma
Aug 4, 2012·Joint, Bone, Spine : Revue Du Rhumatisme·Jean-Marie BerthelotPierre Youinou
Feb 22, 2013·Science Translational Medicine·Fabian Flores-BorjaClaudia Mauri
Apr 10, 2014·Immunological Reviews·Kathleen M CandandoThomas F Tedder
Apr 17, 2014·European Journal of Immunology·Bi-Sheng LiuRene E M Toes
Feb 11, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thomas F Tedder
Apr 23, 2015·Immunity·Elizabeth C Rosser, Claudia Mauri

❮ Previous
Next ❯

Citations

Dec 4, 2020·FEBS Letters·Nadia Arang, J Silvio Gutkind

❮ Previous
Next ❯

Methods Mentioned

BETA
ESR
flow cytometry
density-gradient centrifugation
PMA
PCR
ELISA
Protein Assay

Software Mentioned

GraphPad Prism
CXP Analysis
GraphPad
Image [UNK]
Image Lab

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.