Loss of HCF-1-chromatin association precedes temperature-induced growth arrest of tsBN67 cells

Molecular and Cellular Biology
Joanna WysockaW Herr

Abstract

Human HCF-1 is a large, highly conserved, and abundant nuclear protein that plays an important but unknown role in cell proliferation. It also plays a role in activation of herpes simplex virus immediate-early gene transcription by the viral regulatory protein VP16. A single proline-to-serine substitution in the HCF-1 VP16 interaction domain causes a temperature-induced arrest of cell proliferation in hamster tsBN67 cells and prevents transcriptional activation by VP16. We show here that HCF-1 is naturally bound to chromatin in uninfected cells through its VP16 interaction domain. HCF-1 is chromatin bound in tsBN67 cells at permissive temperature but dissociates from chromatin before tsBN67 cells stop proliferating at the nonpermissive temperature, suggesting that loss of HCF-1 chromatin association is the primary cause of the temperature-induced tsBN67 cell proliferation arrest. We propose that the role of HCF-1 in cell proliferation is to regulate gene transcription by associating with a multiplicity of DNA-bound transcription factors through its VP16 interaction domain.

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Citations

Nov 3, 2009·Apoptosis : an International Journal on Programmed Cell Death·Yiannis P NiniosThomae G Sourlingas
Apr 13, 2011·Cell Biochemistry and Biophysics·Ziad M Eletr, Keith D Wilkinson
Jun 27, 2003·Trends in Biochemical Sciences·Joanna Wysocka, Winship Herr
Jul 3, 2003·Journal of the American Society for Mass Spectrometry·Yuzuru ShiioRuedi Aebersold
Jan 12, 2011·Immunology and Cell Biology·Arnold R SienerthMatthias Becker
Jun 12, 2012·Nature Genetics·Samuel Peña-LlopisJames Brugarolas
Jun 15, 2010·Nature Structural & Molecular Biology·Selma El Messaoudi-AubertGordon Peters
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Jan 8, 2010·The Journal of Biological Chemistry·Cécile E MalnouIsabelle Jariel-Encontre
Oct 10, 2009·The Journal of Biological Chemistry·Yuichi J MachidaAnindya Dutta
Jul 24, 2012·The Journal of Biological Chemistry·Maria VogelauerSiavash K Kurdistani
Feb 18, 2011·Thyroid : Official Journal of the American Thyroid Association·Mylène BrunelleMarie-France Langlois
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