PMID: 9542513Apr 18, 1998Paper

Loss of heterozygosity at microsatellite marker sites for tumour suppressor genes in oesophageal adenocarcinoma

European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology
R J MorganD Alderson

Abstract

Loss of cell cycle control is an important step in the development of human tumours. A number of tumour suppressor genes are involved in cell cycle control, including p16, p53 and Rb. The aim of this study was to seek evidence of deletions of these genes in oesophageal adenocarcinoma. Paired (tumour and normal squamous epithelium) frozen tissue samples from 12 patients were analysed by polymerase chain reaction (PCR) for loss of heterozygosity (LoH) at five microsatellite marker sites (two each for p16 and Rb, one for p53). Aneuploid tumour cell populations were sorted by flow cytometry prior to PCR, to eliminate stromal cell contamination. Of the 12 tumours, 11 (92%) had LoH at one or more loci. LoH at the p53 locus occurred in nine of 12 tumours, at one or both p16 loci in seven of 11 tumours, and at one or both Rb loci in eight of 12 tumours. Five tumours had LoH at two tumour suppressor gene loci, and a further four tumours had LoH at loci for all three genes. Allelic deletions of p53, p16 and Rb are common in oesophageal adenocarcinoma, and may be important in the development of this disease.

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Citations

Feb 27, 2001·Annals of Surgery·B P WijnhovenW N Dinjens
Oct 7, 2006·Current Opinion in Gastroenterology·T Lerut, G Decker
Dec 13, 2002·International Journal of Cancer. Journal International Du Cancer·Dilek GünerPeter T Daniel
Nov 22, 2005·Journal of Surgical Oncology·Linetta B KoppertWinand N M Dinjens

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