Apr 4, 2017

Loss of Heterozygosity Drives Adaptation in Hybrid Yeast

Molecular Biology and Evolution
Caiti S Smukowski HeilMaitreya J Dunham

Abstract

Hybridization is often considered maladaptive, but sometimes hybrids can invade new ecological niches and adapt to novel or stressful environments better than their parents. The genomic changes that occur following hybridization that facilitate genome resolution and/or adaptation are not well understood. Here, we examine hybrid genome evolution using experimental evolution of de novo interspecific hybrid yeast Saccharomyces cerevisiae × Saccharomyces uvarum and their parentals. We evolved these strains in nutrient-limited conditions for hundreds of generations and sequenced the resulting cultures identifying numerous point mutations, copy number changes, and loss of heterozygosity (LOH) events, including species-biased amplification of nutrient transporters. We focused on a particularly interesting example, in which we saw repeated LOH at the high-affinity phosphate transporter gene PHO84 in both intra- and interspecific hybrids. Using allele replacement methods, we tested the fitness of different alleles in hybrid and S. cerevisiae strain backgrounds and found that the LOH is indeed the result of selection on one allele over the other in both S. cerevisiae and the hybrids. This is an example where hybrid genome resolution is d...Continue Reading

Mentioned in this Paper

PHO84
Genome
Genes
Saccharomyces cerevisiae allergenic extract
Nucleic Acid Hybridization Procedure
Inorganic Phosphate Transporter
Environment
Hybridization, Intraspecies
Yeasts
Loss of Heterozygosity

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