Loss of heterozygosity of Kras2 gene on 12p12-13 in Chinese colon carcinoma patients

World Journal of Gastroenterology : WJG
Jun WanPo Zhao

Abstract

To study the loss of heterozygosity (LOH) on 12p12-13 in Chinese colon carcinoma patients. DNA was extracted from 10 specimens of cancer tissue, 10 specimens of adjacent tissue and 10 specimens of normal tissue, respectively. LOH of Kras2 gene was analyzed by polymerase chain reaction (PCR) and denaturing polyacrylamide gel electrophoresis using 11 microsatellite markers on 12p-12-13. LOH of Kras gene was detected at least on one marker of 12p-12-13 in 30% (3/10) of adjacent tissue specimens. The highest frequency of LOH was identified on D12S1034 in 28.57% (2/7) of adjacent tissue specimens. LOH was detected at least on one marker of 12p12-13 in 60% (6/10) of carcinoma tissue specimens, the most frequent LOH was found on D12S1034 and D12S1591 in 42.86% (3/7) of carcinoma tissue specimens. LOH was detected in 30% (3/10) of carcinoma tissue specimens, 30% (3/10) of adjacent tissue specimens, and no signal in 1% (1/0) carcinoma tissue specimen. The occurrence of LOH did not correlate with sex, age, tumor size and lymph node metastasis. Genomic instability may occur on 12p-12-13 of Kras2 gene in the development and progression of colon carcinoma. The high LOH of Kras2 gene may directly influence the transcription and translation o...Continue Reading

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Citations

Mar 18, 2008·Nature·Kian-Huat LimChristopher M Counter
Jan 1, 2008·Forensic Science, Medicine, and Pathology·Karen Page, Eleanor A M Graham
May 19, 2010·Biomarkers in Medicine·Hirendra Nath Banerjee, Mukesh Verma
Sep 26, 2014·World Journal of Gastroenterology : WJG·Branislav RovcaninPetar Ivanovski

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