PMID: 9420034Jan 7, 1998Paper

Loss of intrinsic aminoglycoside resistance in Acinetobacter haemolyticus as a result of three distinct types of alterations in the aac(6')-Ig gene, including insertion of IS17

Antimicrobial Agents and Chemotherapy
E RudantThierry Lambert

Abstract

The distribution of the aac(6')-Ig gene, encoding aminoglycoside 6'-N-acetyltransferase-Ig [AAC(6')-Ig], was studied in 96 Acinetobacter haemolyticus strains and 12 proteolytic Acinetobacter strains, including Acinetobacter genomospecies 6, 13, and 14 and 3 unnamed species assigned to this genomic group by DNA-DNA hybridization. This gene was detected by DNA-DNA hybridization in all 96 A. haemolyticus strains and by PCR in 95 strains but was not detected in strains of other species, indicating that it may be used to identify A. haemolyticus. Three A. haemolyticus strains were susceptible to tobramycin and did not produce an aminoglycoside 6'-N-acetylating activity, although they contained aac(6')-Ig-related sequences. An analysis of three susceptible A. haemolyticus strains indicated that aminoglycoside resistance was abolished by the following three distinct mechanisms: (i) a point mutation in aac(6')-Ig that led to a Met56-->Arg substitution, which was shown by analysis of a revertant to be responsible for the loss of resistance; (ii) a polythymine insertion that altered the reading frame; and (iii) insertion of IS17, a new member of the IS903 family. These observations indicated that AAC(6')-Ig is not essential for the viabi...Continue Reading

References

Jan 1, 1975·Methods in Enzymology·M J Haas, J E Dowding
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Jul 1, 1992·Antimicrobial Agents and Chemotherapy·K J ShawP Downey
Mar 11, 1991·Nucleic Acids Research·T A Holton, M W Graham
May 1, 1989·Research in Microbiology·P J Bouvet, S Jeanjean
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J C MakrisW S Reznikoff
Sep 1, 1987·Annales De L'Institut Pasteur. Microbiology·P J Bouvet, P A Grimont
May 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·B Low
May 14, 1969·Journal of Molecular Biology·H W Boyer, D Roulland-Dussoix
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·N D Grindley, C M Joyce
Dec 1, 1994·Antimicrobial Agents and Chemotherapy·M C PloyT Lambert
Sep 1, 1993·Antimicrobial Agents and Chemotherapy·Y CostaP Courvalin

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Citations

Jan 16, 2007·Clinical Microbiology Reviews·Florence DepardieuPatrice Courvalin
Jun 19, 2003·Journal of Bacteriology·Dariusz BartosikJadwiga Baj
Dec 5, 2018·World Journal of Microbiology & Biotechnology·Heba Shehta SaidRamadan Hassan Ibrahim
Mar 6, 1999·Current Opinion in Microbiology·K Bush, G H Miller
Sep 24, 2004·Journal of the American Chemical Society·Evgeny A FadeevJohn T Groves
Feb 11, 2005·Journal of the American Chemical Society·Minkui LuoJohn T Groves

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