Abstract
L-selectin, an adhesion molecule expressed on the surface of peripheral blood neutrophils, mediates the rolling of neutrophils along vascular endothelium. Stimulation of neutrophils in vitro causes decreased expression of L-selectin on the surface of neutrophils due to shedding. In this study, we have demonstrated that human exudative neutrophils isolated from both skin lesions and from pus exhibit little expression of L-selectin. Although a dramatic accumulation of exudative neutrophils is observed in the skin lesions within 24 hr, there is no accompanying increase in soluble L-selectin. In addition, the levels of soluble L-selectin in the extracellular tissue fluid (97.2 +/- 12.7 micrograms/ml, n = 8) are only 20% of that observed in peripheral plasma (482.4 +/- 35.9 micrograms/ml). These data suggest that shedding of L-selectin from the surface of neutrophils occurs in the peripheral circulation as a prelude to diapedesis of neutrophils into peripheral tissues.
Citations
Apr 24, 2002·Antioxidants & Redox Signaling·Steve D SwainMark T Quinn
Aug 22, 2003·Critical Care : the Official Journal of the Critical Care Forum·Andrew J E SeelyNicolas V Christou
Dec 2, 2011·Journal of Leukocyte Biology·Kol A Zarember, Douglas B Kuhns
Jul 17, 2010·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Thomas U MarronCharlotte Cunningham-Rundles
Apr 11, 2013·Journal of Immunological Methods·Lisa DavidssonJohan Bylund
Dec 21, 2018·Journal of Leukocyte Biology·Lena BjörkmanJohan Bylund
Jun 1, 1997·European Journal of Immunology·U M Walter, A C Issekutz
Jun 11, 2005·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Julie U HolleC Mark Taylor
Sep 17, 2009·Inflammation·Boris K Pliyev, Mikhail Yu Menshikov
Aug 5, 1998·International Journal of Clinical & Laboratory Research·A D CrockardS A Hawkins
Nov 18, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gustavo Batista MenezesPaul Kubes
Nov 5, 2019·Frontiers in Immunology·Elin BernsonFredrik B Thorén
Aug 12, 2019·Journal of Dairy Science·E J PutzJ D Lippolis
Feb 13, 2021·Pharmaceuticals·João F S RodriguesElizabeth S Fernandes
Jan 5, 2000·Journal of Immunological Methods·P Follin
Nov 24, 1999·Infection and Immunity·J Soltys, M T Quinn