Loss of LIN-35, the Caenorhabditis elegans ortholog of the tumor suppressor p105Rb, results in enhanced RNA interference

Genome Biology
Ben LehnerAndrew G Fraser

Abstract

Genome-wide RNA interference (RNAi) screening is a very powerful tool for analyzing gene function in vivo in Caenorhabditis elegans. The effectiveness of RNAi varies from gene to gene, however, and neuronally expressed genes are largely refractive to RNAi in wild-type worms. We found that C. elegans strains carrying mutations in lin-35, the worm ortholog of the tumor suppressor gene p105Rb, or a subset of the genetically related synMuv B family of chromatin-modifying genes, show increased strength and penetrance for many germline, embryonic, and post-embryonic RNAi phenotypes, including neuronal RNAi phenotypes. Mutations in these same genes also enhance somatic transgene silencing via an RNAi-dependent mechanism. Two genes, mes-4 and zfp-1, are required both for the vulval lineage defects resulting from mutations in synMuv B genes and for RNAi, suggesting a common mechanism for the function of synMuv B genes in vulval development and in regulating RNAi. Enhanced RNAi in the germline of lin-35 worms suggests that misexpression of germline genes in somatic cells cannot alone account for the enhanced RNAi observed in this strain. A worm strain with a null mutation in lin-35 is more sensitive to RNAi than any other previously desc...Continue Reading

References

Mar 1, 1981·Developmental Biology·M Chalfie, J Sulston
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·V SahaB D Young
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Nathaniel R DudleyBob Goldstein
Jun 14, 2002·Current Biology : CB·Mike Boxem, Sander van den Heuvel
Aug 15, 2002·Current Biology : CB·Femke SimmerRonald H A Plasterk
Oct 19, 2002·Mechanisms of Development·Yun Zhang, Martin Chalfie
May 6, 2004·Journal of Cell Science·Maxim V Frolov, Nicholas J Dyson
May 28, 2004·Briefings in Functional Genomics & Proteomics·Ben LehnerChristopher M Sanderson
Jun 16, 2004·Developmental Biology·David S FayBethany L Johanson
Sep 17, 2004·Nature·Craig C Mello, Darryl Conte
Nov 17, 2004·Genes & Development·Peter W LewisMichael R Botchan
Dec 3, 2004·Genes & Development·Joseph S Lipsick
Mar 26, 2005·Science·John K KimGary Ruvkun
Jul 29, 2005·Nature·Derek SieburthJoshua M Kaplan
Mar 14, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yosuke MatsubaraYuji Yamanashi
Nov 10, 2007·Nucleic Acids Research·Anthony RogersPaul W Sternberg

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Citations

Jun 1, 2010·Nature Methods·Andrea CalixtoMartin Chalfie
Aug 23, 2008·Nature Reviews. Molecular Cell Biology·Sander van den Heuvel, Nicholas J Dyson
Dec 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Alla GrishokPhillip A Sharp
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Dona C LoveJohn A Hanover
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alexander BounoutasMartin Chalfie
Oct 17, 2012·DNA and Cell Biology·Julie M Claycomb
May 1, 2008·Briefings in Functional Genomics & Proteomics·Ben Lehner, Insuk Lee
May 1, 2007·BMC Developmental Biology·Jimmy Ouellet, Richard Roy
Jun 6, 2008·Genome Biology·Stephen E Von Stetina, Susan E Mango
Apr 26, 2008·PLoS Genetics·Natalia V KirienkoDavid S Fay
May 19, 2011·PLoS Genetics·Tomoko M TabuchiKirsten A Hagstrom
Mar 14, 2012·PLoS Genetics·Katlin B MassirerAmy E Pasquinelli
Sep 2, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jóhanna ArnadóttirMartin Chalfie
Sep 24, 2013·Worm·Katlin B Massirer, Amy E Pasquinelli
Jan 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Caroline SchmitzHarald Hutter
Mar 21, 2007·Developmental Biology·Natalia V Kirienko, David S Fay
Dec 23, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Brandon Praslicka, Chris R Gissendanner
Jan 1, 2012·Wiley Interdisciplinary Reviews. Developmental Biology·Thomas Boulin, Oliver Hobert
Sep 12, 2018·Current Protocols in Molecular Biology·Sakthimala Jagadeesan, Abdul Hakkim
Feb 23, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Natalia V KirienkoDavid S Fay
Mar 7, 2019·Nucleic Acids Research·Evandro A De-SouzaMarcelo A Mori
Jan 23, 2015·EMBO Reports·Anikó I NagyHoward A Baylis
Jan 7, 2015·Current Protocols in Molecular Biology·Darryl ConteCraig C Mello
Apr 17, 2007·Developmental Biology·David S Fay, John Yochem
Feb 22, 2012·Cell·Arun K RamaniAndrew G Fraser

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Methods Mentioned

BETA
dissecting

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