Loss of maternal EED results in postnatal overgrowth

Clinical Epigenetics
Lexie ProkopukPatrick S Western

Abstract

Investigating how epigenetic information is transmitted through the mammalian germline is the key to understanding how this information impacts on health and disease susceptibility in offspring. EED is essential for regulating the repressive histone modification, histone 3 lysine 27 tri-methylation (H3K27me3) at many developmental genes. In this study, we used oocyte-specific Zp3-Cre recombinase (Zp3Cre) to delete Eed specifically in mouse growing oocytes, permitting the study of EED function in oocytes and the impact of depleting EED in oocytes on outcomes in offspring. As EED deletion occurred only in growing oocytes and females were mated to normal wild type males, this model allowed the study of oocyte programming without confounding factors such as altered in utero environment. Loss of EED from growing oocytes resulted in a significant overgrowth phenotype that persisted into adult life. Significantly, this involved increased adiposity (total fat) and bone mineral density in offspring. Similar overgrowth occurs in humans with Cohen-Gibson (OMIM 617561) and Weaver (OMIM 277590) syndromes, that result from de novo germline mutations in EED or its co-factor EZH2, respectively. Consistent with a role for EZH2 in human oocytes,...Continue Reading

References

Jun 3, 2000·Nature Genetics·M BarnaP P Pandolfi
Jun 8, 2001·Molecular and Cellular Biology·D O'CarrollT Jenuwein
Dec 6, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·J J Eppig
Dec 24, 2002·Nature Immunology·I-Hsin SuAlexander Tarakhovsky
Oct 20, 2006·Development·Waldemar J Racki, Joel D Richter
Apr 21, 2007·Journal of Internal Medicine·D J P Barker
Apr 7, 2009·American Journal of Human Genetics·Helen V FirthNigel P Carter
Jun 16, 2009·Nature·Saher Sue HammoudBradley R Cairns
May 18, 2010·Nature Structural & Molecular Biology·Urszula BrykczynskaAntoine H F M Peters
Dec 20, 2011·American Journal of Human Genetics·William T GibsonSteven J M Jones
Dec 23, 2011·Oncotarget·Katrina Tatton-BrownNazneen Rahman
Apr 14, 2012·Genes & Development·Eszter PosfaiAntoine H F M Peters
Jan 29, 2013·Developmental Cell·Jia-Hui NgHuck-Hui Ng
Feb 23, 2013·Nature Reviews. Molecular Cell Biology·Rong Li, David F Albertini
Jun 19, 2013·Nature Structural & Molecular Biology·Serap ErkekAntoine H F M Peters
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Bluma J LeschDavid C Page
Jan 25, 2014·Nature Communications·Qi CaoArul M Chinnaiyan
Apr 18, 2014·The Journal of Endocrinology·Peter SmithRaymond J Rodgers
Jul 22, 2014·Clinical and Experimental Reproductive Medicine·Kyeoung-Hwa Kim, Kyung-Ah Lee
Mar 20, 2015·Journal of Human Genetics·Ana S A CohenWilliam T Gibson
May 6, 2015·Seminars in Cell & Developmental Biology·Rachel Stegemann, David A Buchner
Jul 3, 2015·Seminars in Cell & Developmental Biology·Denise S Fernandez-TwinnSusan E Ozanne
Sep 15, 2015·Epigenomics·Lexie ProkopukJessica M Stringer
Nov 26, 2015·Seminars in Cell & Developmental Biology·Kirsten Hogg, Patrick S Western
May 20, 2016·Journal of Human Genetics·Ana Sa Cohen, William T Gibson
Sep 16, 2016·Nature Reviews. Genetics·Peter A JonesStephen Baylin
Nov 22, 2016·American Journal of Medical Genetics. Part a·Erin CooneyLorraine Potocki
Dec 10, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sarah HemmingStan Gronthos
Jan 4, 2017·Human Mutation·Henk P J BuermansTahar van der Straaten
Feb 23, 2017·EMBO Reports·Ajithkumar VasanthakumarRhys S Allan
Feb 24, 2017·Human Mutation·Eri ImagawaNaomichi Matsumoto
May 6, 2017·American Journal of Human Genetics·Katrina Tatton-BrownNazneen Rahman

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Citations

Jan 14, 2019·Reproduction, Fertility, and Development·Elizabeth G BromfieldMichael J Bertoldo
Aug 28, 2019·Nature Communications·Roberta RagazziniRaphaël Margueron
Jan 8, 2019·F1000Research·Ellen G JarredPatrick S Western
Jul 16, 2020·International Journal of Molecular Sciences·Yu-Shan ChenFeng-Sheng Wang
Mar 10, 2020·F1000Research·Hannah Demond, Gavin Kelsey
Jun 13, 2020·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A Tomar, R Teperino
Dec 15, 2020·Nature Communications·Tie-Gang MengQing-Yuan Sun
Dec 17, 2020·International Journal of Molecular Sciences·Kyoung-Tae KimInbo Han
Jun 3, 2021·Genes & Development·Courtney W Hanna, Gavin Kelsey

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Methods Mentioned

BETA
transgenic
ubiquitination
PCR
genetic modifications
X-ray

Software Mentioned

R Core
lmerTest
R
GraphPad Prism
DECIPHER
PRISM
lme4

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