Loss of microRNA-124 expression in neurons in the peri-lesion area in mice with spinal cord injury

Neural Regeneration Research
Yu ZhaoJian Wang

Abstract

MicroRNA-124 (miR-124) is abundantly expressed in neurons in the mammalian central nervous system, and plays critical roles in the regulation of gene expression during embryonic neurogenesis and postnatal neural differentiation. However, the expression profile of miR-124 after spinal cord injury and the underlying regulatory mechanisms are not well understood. In the present study, we examined the expression of miR-124 in mouse brain and spinal cord after spinal cord injury using in situ hybridization. Furthermore, the expression of miR-124 was examined with quantitative RT-PCR at 1, 3 and 7 days after spinal cord injury. The miR-124 expression in neurons at the site of injury was evaluated by in situ hybridization combined with NeuN immunohistochemical staining. The miR-124 was mainly expressed in neurons throughout the brain and spinal cord. The expression of miR-124 in neurons significantly decreased within 7 days after spinal cord injury. Some of the neurons in the peri-lesion area were NeuN(+)/miR-124(-). Moreover, the neurons distal to the peri-lesion site were NeuN(+)/miR-124(+). These findings indicate that miR-124 expression in neurons is reduced after spinal cord injury, and may reflect the severity of spinal cord inj...Continue Reading

References

May 15, 2002·Current Biology : CB·Mariana Lagos-QuintanaThomas Tuschl
Oct 3, 2003·Trends in Neurosciences·Florence M Bareyre, Martin E Schwab
Dec 17, 2005·Stem Cells·Anna M KrichevskyKenneth S Kosik
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Cecilia ConacoGail Mandel
Jun 1, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Monika DeoDavid L Turner
Jun 17, 2006·Annual Review of Neuroscience·Xinwei CaoFred H Gage
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Monica C Vella, Frank J Slack
Apr 1, 2008·Journal of Neurotrauma·Jason R PlemelWolfram Tetzlaff
Mar 17, 2009·Nature Neuroscience·Li-Chun ChengFiona Doetsch
Jul 7, 2009·Experimental Neurology·Nai-Kui LiuXiao-Ming Xu
Sep 25, 2012·Neuroscience Letters·Weiwei XuXiaodan Jiang
Apr 20, 2013·Forensic Science International : Synergy·Cornelius CourtsBurkhard Madea
May 3, 2014·Molecular and Cellular Neurosciences·Victoria M HoKelsey C Martin
Sep 10, 2014·Nature Reviews. Cardiology·Thomas Thum
Oct 16, 2014·Cells·Jane A Leopold

❮ Previous
Next ❯

Citations

Nov 24, 2016·Frontiers in Surgery·Nikolay L MartirosyanNicholas Theodore
Aug 29, 2019·Expert Opinion on Therapeutic Targets·Shuo WangShuxin Li
Sep 13, 2020·The Journal of Comparative Neurology·Jun ChenZhongtao Liu
Apr 23, 2021·Cellular and Molecular Neurobiology·Jinying XuGuangfan Chi

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
antisense oligonucleotide

Software Mentioned

SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.