Loss of pacing-induced preconditioning in rat hearts: role of nitric oxide and cholesterol-enriched diet

Journal of Molecular and Cellular Cardiology
P FerdinandyL Dux

Abstract

We examined whether the inhibition of nitric oxide (NO) synthesis by NG-nitro-L-arginine (lNNA) abolished pacing-induced preconditioning, and if prolonged exposure to cholesterol-enriched diet led to the loss of preconditioning due to decreased cardiac NO formation. Therefore, Wistar rats fed 2% cholesterol-enriched diet or standard diet for 24 weeks were treated with a single dose of 1 mg/kg lNNA or its solvent at the end of the week 24, respectively. Isolated hearts from all groups were subjected to either preconditioning induced by three consecutive periods of pacing at 600 beats/min for 5 min, with 5-min interpacing periods, or time-matched non-preconditioning perfusion, followed by a 10-min coronary occlusion, respectively. In the control group, coronary occlusion after a non-preconditioning protocol decreased aortic flow (AF) from 45.4+/-2.4 to 15.6+/-1.5 ml/min, and resulted in a lactate dehydrogenase (LDH) release of 219+/-55 mU/min/g, however, preconditioning attenuated the consequences of coronary occlusion [AF: 27.3+/-1.7 ml/min (P<0.05); LDH: 44+/-14 mU/min/g (P<0.05)]. Preconditioning did not confer protection in the lNNA-treated (AF: 17.4+/-1.5 ml/min; LDH: 151+/-21 mU/min/g), and/or in the high-cholesterol-fed gr...Continue Reading

Citations

Feb 22, 2005·Basic Research in Cardiology·Guro Valen, Jarle Vaage
Oct 16, 2009·European Journal of Applied Physiology·You-Hua WangWei-Jin Zang
May 26, 2005·Journal of Molecular and Cellular Cardiology·Andreas SkyschallyGerd Heusch
Sep 13, 2003·European Journal of Pharmacology·Tamás CsontPéter Ferdinandy
Dec 28, 1999·European Journal of Pharmacology·J NémethZ Szilvássy
Apr 11, 2002·Acta Anaesthesiologica Scandinavica·Hilchen T Sommerschild, Knut Arvid Kirkebøen
Mar 18, 2004·Antioxidants & Redox Signaling·Bela JuhaszArpad Tosaki
May 25, 2012·Journal of Cardiovascular Pharmacology·László DrimbaBarna Peitl
Jul 23, 1998·Clinical and Experimental Pharmacology & Physiology·P FerdinandyA Tosaki
Jul 23, 1999·Annals of the New York Academy of Sciences·S de ZeeuwP D Verdouw
Feb 1, 2011·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Punate WeerateerangkulNipon Chattipakorn
Apr 7, 2007·Journal of Cardiovascular Medicine·Naranjan S DhallaSuresh K Gupta
Oct 27, 2007·Redox Report : Communications in Free Radical Research·Vladimir VinokurMordechai Chevion
Feb 1, 2002·Biochemical and Biophysical Research Communications·Tamás CsontPéter Ferdinandy
Aug 24, 2012·Canadian Journal of Physiology and Pharmacology·Verónica D'AnnunzioRicardo J Gelpi
Apr 1, 2000·Clinical and Experimental Hypertension : CHE·O JungW Linz
Jul 6, 2010·European Journal of Pharmacology·Harlokesh Narayan YadavPyare Lal Sharma
Oct 13, 2009·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·R HuhnN C Weber
Jan 21, 2016·Oxidative Medicine and Cellular Longevity·Csaba CsonkaTamás Csont
Jul 14, 2007·European Journal of Pharmacology·László CzakóJános Lonovics
May 8, 2007·International Journal of Cardiology·Rafal DworakowskiAndrzej Rynkiewicz
Jul 6, 2014·British Journal of Pharmacology·C CsonkaT Csont
Feb 28, 2003·The Annals of Thoracic Surgery·Jarle Vaage, Guro Valen
Oct 10, 2014·British Journal of Pharmacology·O PechánováP Ferdinandy
Aug 22, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Johan S EsterhuyseEugene F du Toit
Nov 20, 2015·Cardiovascular Diabetology·Tamás BaranyaiZoltán Giricz
Apr 20, 2001·The Annals of Thoracic Surgery·G LiG Valen
Feb 25, 2003·British Journal of Pharmacology·Péter Ferdinandy, Richard Schulz
Feb 14, 2015·Journal of Cellular and Molecular Medicine·Mücella KircaRainer Schulz
May 27, 2014·Circulation Journal : Official Journal of the Japanese Circulation Society·Giampaolo NiccoliFilippo Crea

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