Loss of phosphotyrosine phosphatase activity and changes in the tyrosine phosphorylation state of proteins after storage of sheep platelets in plasma or Seto solution at 4 degrees C

Vox Sanguinis
A Hernández-HernándezJ Sánchez-Yagüe

Abstract

During platelet storage an array of deleterious changes occur, through mechanisms not fully understood, which impair platelet haemostasis. Transfused platelets should maintain the integrated networks of signalling pathways that regulate platelet activation and functionality. We hypothesized that protein phosphorylation and dephosphorylation, which play a fundamental role in these pathways, might be affected by platelet storage. We therefore investigated whether the activity of phosphotyrosine phosphatase (PTP), which belongs to an oxidant-susceptible group of enzymes involved in the platelet signal-transduction pathways that ensure platelet functionality, is affected by platelet storage. Using sheep platelet species as a model system, we conducted serial studies on the membranes of platelets and microparticles shed during platelet storage, in their own plasma or in a synthetic medium called Seto, for up to 5 days at 4 degrees C. A progressive decrease in both total and specific membrane-associated PTP activities from whole platelets (but not from microparticles) located within each platelet storage bag was observed from day 1 onwards in both types of storage media. These decreases could be partly avoided by the addition of vita...Continue Reading

References

Sep 1, 1992·Biulleten' eksperimental'noĭ biologii i meditsiny·V A AlmazovB G Bershadskiĭ
Jan 1, 1991·Life Sciences·H M SmilowitzP M Epstein
Apr 1, 1997·Transfusion Medicine Reviews·J Seghatchian, P Krailadsiri
Jul 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·A Hernández-HernándezM Llanillo
Jun 25, 1999·Free Radical Biology & Medicine·A Hernández-HernándezM Llanillo
Jul 17, 1999·Biochimica Et Biophysica Acta·A Hernández-HernándezJ Sánchez-Yagüe
Oct 3, 1999·Archives of Pathology & Laboratory Medicine·S Silberman
Oct 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·F WROBLEWSKI, J S LADUE

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