Loss of Porcupine impairs convergent extension during gastrulation in zebrafish

Journal of Cell Science
Qiuhong ChenShinji Takada

Abstract

Porcupine (Porcn), an O-acyltransferase located in the endoplasmic reticulum (ER), is required for lipidation of Wnt proteins to enable their trafficking from the ER in mammalian cell culture. However, it is unclear whether Porcn is required for trafficking of all members of the Wnt family. In this study, we investigated the function of Porcn in zebrafish embryos. We identified two zebrafish homologs of porcupine, porcn and porcupine-like (porcn-l). Zebrafish porcn, but not porcn-l, restores secretion of Wnt proteins in porcn-deficient mouse L cells. Morpholino-mediated knockdown of porcn in zebrafish embryos impairs convergence and extension (CE) during gastrulation without changing embryonic patterning. Moreover, porcn interacts genetically with wnt5b and wnt11 in regulating CE. By contrast, porcn-deficient embryos do not exhibit phenotypes caused by failure in canonical Wnt signaling, which is activated by several Wnt ligands, including Wnt3a. Furthermore, expression of genes regulated by the canonical Wnt signaling pathway is not perturbed in knockdown embryos relative to that in controls. Although the trafficking and lipidation of ectopically expressed zebrafish Wnt5b and mouse Wnt5a are impaired in porcn-deficient embryos...Continue Reading

References

Jan 6, 1994·Nature·E SiegfriedN Perrimon
Aug 1, 1995·Mechanisms of Development·A R UngarR T Moon
Jul 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C B KimmelT F Schilling
May 16, 2000·Nature·J B WallingfordR M Harland
Jun 24, 2000·European Journal of Biochemistry·K TanakaT Kadowaki
Jul 10, 2002·Nature Cell Biology·Jason R JessenLilianna Solnica-Krezel
Feb 20, 2003·Developmental Biology·Arne C LekvenRandall T Moon
Apr 5, 2003·Mechanisms of Development·Beate KilianCarl Philipp Heisenberg
Jul 23, 2003·Development·Filipa Carreira-BarbosaMasazumi Tada
Sep 4, 2003·The Journal of Cell Biology·Trudi A WestfallDiane C Slusarski
May 19, 2004·Mechanisms of Development·Gerri R BucklesArne C Lekven
Jan 5, 2005·Genes & Development·Takaaki MatsuiJuan Carlos Izpisúa Belmonte
Apr 29, 2006·Science·Damien Y M CoudreuseHendrik C Korswagen
May 9, 2006·Cell·Kerstin BartschererMichael Boutros
Nov 17, 2006·Development·Robyn M GoodmanErica M Selva
Dec 5, 2006·Developmental Cell·Ritsuko TakadaShinji Takada
May 29, 2007·PLoS Genetics·Mara E RobuStephen C Ekker
Jun 5, 2007·Nature Genetics·Xiaoling WangIgnatia B Van den Veyver
Jun 5, 2007·Nature Genetics·Karl-Heinz GrzeschikRudolf Happle
Dec 28, 2007·Developmental Cell·Chun-Liang PanGian Garriga
Dec 28, 2007·Developmental Cell·Pei-Tzu YangHendrik C Korswagen
Jan 15, 2008·Nature Cell Biology·Fillip PortKonrad Basler
Jan 15, 2008·Nature Cell Biology·Xavier Franch-MarroJean-Paul Vincent
Apr 24, 2008·Journal of Cell Science·Xavier Franch-MarroJean-Paul Vincent
Apr 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jesus A GutierrezJohn E Hale
Jun 16, 2010·Genome Research·Brian J CoxJanet Rossant
Sep 10, 2010·Journal of Cell Science·Gary S CoombsDavid M Virshup
May 11, 2011·Developmental Biology·Steffen BiecheleJanet Rossant

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Citations

Jan 6, 2015·Nature Communications·Eliana StanganelloSteffen Scholpp
Aug 17, 2018·ELife·Chengyong ShenLin Mei
Jan 6, 2017·Journal of Biochemistry·Shinji TakadaYusuke Mii
Aug 14, 2012·The Journal of Biological Chemistry·Kyle D Proffitt, David M Virshup
Apr 8, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Debdut NaskarMalini Sen
Mar 5, 2018·Journal of Cellular and Molecular Medicine·Hsiu-Kuan LinMing-Jer Tang
Mar 19, 2016·Birth Defects Research. Part C, Embryo Today : Reviews·Yoh WadaJyunichiro Yasukawa
Jun 14, 2013·Development·Steffen BiecheleJanet Rossant
May 18, 2012·The Journal of Biological Chemistry·Michael E DodgeLawrence Lum

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