Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer

International Journal of Oncology
Hui ZhangDechun Li

Abstract

Profilin 2 (PFN2) functions as an actin cytoskeleton regulator and serves an important role in cell motility. However, a role for PFN2 in the progression of colorectal cancer (CRC), particularly in metastasis, has yet to be clarified. The aim of the present study was to investigate whether PFN2 served specific roles in the progression of human CRC. The results demonstrated that PFN2 was differentially expressed in CRC tissues and cell lines by reverse transcription-quantitative polymerase chain reaction and western blotting. PFN2 expression was also negatively associated with the degree of tumor metastasis. Low PFN2 expression in CRC cells was related with enhanced epithelial-mesenchymal transition (EMT) and, in turn, may increase migratory capabilities. Overexpression of PFN2 in CRC cell lines with a low level of endogenous PFN2 inhibited the EMT process, as well as the associated migration; in addition, myosin light chain (MLC) phosphorylation was upregulated. Inhibition of MLC phosphorylation attenuated the inhibition of EMT and cell migratory abilities induced by PFN2 overexpression in CRC cell lines, the results suggested that PFN2 may suppress cancer EMT and the subsequent metastasis by regulating cytoskeletal reorganizat...Continue Reading

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Citations

Jul 15, 2020·Biophysics Reviews·Rhonda J Davey, Pierre Dj Moens
Nov 12, 2020·Biology·Jaya Aseervatham
Mar 7, 2021·Biomolecules·Roberta MelchionnaPaola Nisticò
Dec 18, 2021·Frontiers in Cell and Developmental Biology·ShiJian DingYu-Dong Cai

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Methods Mentioned

BETA
transfection
scraping

Software Mentioned

ImageJ
Statistical Package for Social Science
SPSS
NIS
Elements
Adobe Photoshop

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