Loss of repression of HuR translation by miR-16 may be responsible for the elevation of HuR in human breast carcinoma

Journal of Cellular Biochemistry
Fang XuWengong Wang

Abstract

Elevated levels of RNA binding protein HuR were found in various human cancers. However, the mechanisms underlying HuR over-expression in cancers have not been fully elucidated. Here, we show that miR-16 acts as a novel post-transcriptional regulator for HuR. Knockdown of miR-16 increased HuR protein levels in MDA-MB-231 cells, while over-expression of pre-miR16 reduced HuR expression. Neither knockdown nor over-expression of miR-16 could alter the mRNA levels of HuR. Instead, knockdown of miR-16 increased the level of de novo synthesized HuR protein. Importantly, mechanistic studies showed that miR-16 associated with the 3'UTR of HuR, and knockdown of miR-16 markedly increased the luciferase activity of a HuR 3'UTR-containing reporter. We further demonstrate that the level of miR-16 was inversely correlated with HuR protein level in human breast carcinoma. Together, our results suggest an important role of miR-16 in regulating HuR translation and link this regulatory pathway to human breast cancer.

References

Apr 16, 1998·The Journal of Biological Chemistry·N S LevyA P Levy
Jan 11, 2000·Molecular and Cellular Biology·W WangM Gorospe
Oct 6, 2000·The Journal of Cell Biology·C M BrennanJ A Steitz
Dec 26, 2001·The Journal of Clinical Investigation·D A DixonS M Prescott
Apr 22, 2003·The Journal of Biological Chemistry·Sibani SenguptaTimothy Hla
Jun 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Krystyna Mazan-MamczarzMyriam Gorospe
Aug 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Carsten DenkertSteffen Hauptmann
Jan 14, 2005·Journal of Cellular Physiology·Arianna BottoniEttore C degli Uberti
Sep 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Amelia CimminoCarlo M Croce
Aug 17, 2006·Cancer Research·Xun Guo, Rebecca S Hartley
Feb 24, 2007·Molecular Cell·Kotb AbdelmohsenMyriam Gorospe
Mar 8, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gopalan NatarajanShrikant Anant
Mar 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·George A CalinCarlo M Croce
Dec 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kotb AbdelmohsenMyriam Gorospe
Jul 4, 2009·Genes & Development·Hyeon Ho KimMyriam Gorospe
Oct 31, 2009·RNA Biology·Xu GuoRebecca S Hartley

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Citations

Oct 19, 2011·Cancer Metastasis Reviews·Ashleigh E MooreDan A Dixon
Apr 23, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhongpeng ZhuJun Wang
Jun 6, 2012·Nature Structural & Molecular Biology·Laure WeillRaúl Méndez
Jun 6, 2012·Comparative and Functional Genomics·Laurence Wurth
Dec 12, 2012·PloS One·Noelia Agra AndrieuPaloma Martín-Sanz
Aug 13, 2014·Medical Oncology·Ioly Kotta-LoizouStamatios Theocharis
Sep 6, 2013·Nature Communications·Virginie Dormoy-RacletImed-Eddine Gallouzi
Apr 2, 2015·International Journal of Molecular Sciences·Xun GuoRebecca S Hartley
Aug 21, 2014·Genes·Lorna W Harries
Aug 12, 2014·Science China. Life Sciences·WenGong Wang
Mar 1, 2016·Mechanisms of Ageing and Development·Yujia YuanYanrong Lu
Jun 8, 2012·Journal of Cellular Physiology·Mamata SinghBeth S Lee
Feb 4, 2014·International Journal of Cancer. Journal International Du Cancer·Jie HuJiping Wang
Jul 15, 2015·Vascular Cell·Dorina VeliceasaOlga V Volpert
May 28, 2015·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Charlène VigourouxGuillaume Gauchotte
Sep 16, 2011·Blood·Maria BaouJohn J Murphy
Sep 4, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Dandan WangXiaoyu Yan
Nov 11, 2015·Scientific Reports·Vito Giuseppe D'AgostinoAlessandro Provenzani
May 15, 2013·International Journal of Molecular Sciences·Jun WangBaocheng Wang
Nov 18, 2017·Nucleic Acids Research·Domenica MarcheseUNKNOWN Catalan MSA Registry (CMSAR)
Nov 2, 2018·Current Cancer Drug Targets·Mingxia WuWilliam C S Cho
Jan 16, 2020·American Journal of Physiology. Cell Physiology·Qian LiChen Huang
Jan 17, 2014·American Journal of Physiology. Renal Physiology·Rudolf Pullmann, Hamid Rabb
Aug 14, 2019·Scientific Reports·Phillip B MunsonArti Shukla
Aug 14, 2020·International Journal of Molecular Sciences·Iwona Rzeszutek, Aditi Singh

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