Loss of signal transduction and inhibition of lymphocyte locomotion in a ground-based model of microgravity

In Vitro Cellular & Developmental Biology. Animal
Alamelu SundaresanNeal R Pellis

Abstract

Inflammatory adherence to, and locomotion through the interstitium is an important component of the immune response. Conditions such as microgravity and modeled microgravity (MMG) severely inhibit lymphocyte locomotion in vitro through gelled type I collagen. We used the NASA rotating wall vessel bioreactor or slow-turning lateral vessel as a prototype for MMG in ground-based experiments. Previous experiments from our laboratory revealed that when lymphocytes (human peripheral blood mononuclear cells [PBMCs]) were first activated with phytohemaglutinin followed by exposure to MMG, locomotory capacity was not affected. In the present study, MMG inhibits lymphocyte locomotion in a manner similar to that observed in microgravity. Phorbol myristate acetate (PMA) treatment of PBMCs restored lost locomotory capacity by a maximum of 87%. Augmentation of cellular calcium flux with ionomycin had no restorative effect. Treatment of lymphocytes with mitomycin C prior to exposure to MMG, followed by PMA, restored locomotion to the same extent as when nonmitomycin C-treated lymphocytes were exposed to MMG (80-87%), suggesting that deoxyribonucleic acid replication is not essential for the restoration of locomotion. Thus, direct activation o...Continue Reading

References

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Citations

Oct 30, 2008·Cell Communication and Signaling : CCS·Oliver UllrichKerstin Lang
Jul 18, 2009·In Vitro Cellular & Developmental Biology. Animal·Wendy FitzgeraldJean-Charles Grivel
Dec 15, 2010·International Journal of Radiation Biology·Asma RizviDaila S Gridley
Jan 28, 2006·Biotechnology and Bioengineering·Kelly JohansonTimothy G Hammond
May 12, 2009·Annals of the New York Academy of Sciences·Alamelu Sundaresan, Neal R Pellis
Jun 24, 2006·Journal of Cellular Biochemistry·Nancy E WardDiana Risin
Jan 20, 2011·Cytoskeleton·Maria Antonia MeloniMarianne Cogoli-Greuter
Jul 23, 2009·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·Leonardo K MartinelliAlamelu Sundaresan
Feb 5, 2015·BioMed Research International·Fiona LouisAlain Guignandon
Oct 14, 2014·BioMed Research International·Teresa AdellGennaro Auletta
Feb 24, 2006·European Biophysics Journal : EBJ·Massimo GalimbertiFrancesco S Pavone
Feb 18, 2004·Journal of Applied Physiology·A SundaresanN R Pellis
Jul 20, 2006·In Vitro Cellular & Developmental Biology. Animal·Maureen A Morrow
Mar 9, 2006·Radiation and Environmental Biophysics·Lorenzo Manti
Oct 23, 2002·Journal of Applied Physiology·Kelly JohansonTimothy G Hammond
Jul 13, 2006·International Journal of Immunopathology and Pharmacology·J A M Maier
Jul 1, 2020·BioMed Research International·Zhonghong CaoXiufang Zhang

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Methods Mentioned

BETA
PMA

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