Loss of sphingosine 1-phosphate receptor 3 gene function impairs injury-induced stromal angiogenesis in mouse cornea.

Laboratory Investigation; a Journal of Technical Methods and Pathology
Shingo YasudaShizuya Saika

Abstract

Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid generated through sphingosine kinase1 (SPK1)-mediated phosphorylation of sphingosine. We show here that injury-induced S1P upregulation increases corneal neovascularization through stimulating S1PR3, a cognate receptor. since this response was suppressed in S1PR3-knockout mice. Furthermore, Cayman10444, a selective S1PR3 inhibitor, reduced this response in WT mice. Such reductions in neovascularization were associated with reduced vascular endothelial growth factor A (VEGF-A) mRNA expression levels in WT TKE2 corneal epithelial cells and macrophages treated with CAY10444 as well as macrophages isolated from S1PR3 KO mice. S1P increased tube-like vessel formation in human vascular endothelial cells (HUVEC) and human retinal microvascular endothelial cells (HRMECs) cells expressing S1PR3. In S1PR3 KO mice, TGFβ1-induced increases in αSMA gene expression levels were suppressed relative to those in the WT counterparts. In S1PR3 deficient macrophages, VEGF-A expression levels were lower than in WT macrophages. Transforming growth factor β1(TGFβ1) upregulated SPK1 expression levels in ocular fibroblasts and TKE2 corneal epithelial cells. CAY10444 blocked S1P-induced increases ...Continue Reading

References

May 3, 2003·Nature Reviews. Molecular Cell Biology·Sarah Spiegel, Sheldon Milstien
Jun 11, 2004·Annual Review of Biochemistry·Isao IshiiJerold Chun
Jul 24, 2004·Seminars in Cell & Developmental Biology·Timothy Hla
Jun 27, 2007·Investigative Ophthalmology & Visual Science·Shuko FujitaYoshitaka Ohnishi
Oct 27, 2007·Investigative Ophthalmology & Visual Science·Ai KitanoPeter S Reinach
Mar 6, 2008·Journal of Cellular and Molecular Medicine·Tetsuya KawakitaScheffer C G Tseng
Mar 1, 2008·Clinical Ophthalmology·Martin S SpitzerPeter Szurman
May 4, 2011·World Journal of Biological Chemistry·Yoh TakuwaKazuaki Yoshioka
Jun 7, 2011·The American Journal of Pathology·Yuka OkadaShizuya Saika
Jun 8, 2012·BioFactors·Yoh TakuwaNoriko Takuwa
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Anthony O AwojooduEdward Botchwey
Jun 7, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Karl QuintMalgorzata Anna Kolodziej
Nov 26, 2014·Cell Adhesion & Migration·Julie Gavard
Dec 3, 2014·Advances in Biological Regulation·Long Shuang Huang, Viswanathan Natarajan
Mar 8, 2016·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hiroki IwanishiShizuya Saika
Jun 28, 2016·General and Comparative Endocrinology·C G Hernández-CoronadoA M Rosales-Torres
Mar 29, 2019·Biomolecules & Therapeutics·Jae-Yeong Heo, Dong-Soon Im
Mar 21, 2020·Frontiers in Cell and Developmental Biology·Raj Kumar SahShailja Singh

❮ Previous
Next ❯

Citations

Apr 4, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Qian LiGuanghui Yi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.