Loss of spinal motor neurons and alteration of alpha-synuclein immunostaining in MPTP induced Parkinsonism in mice

Journal of Chemical Neuroanatomy
Giorgio VivacquaFrancesco Fornai

Abstract

1-Methyl, 4-phenyl, 1,2,3,6-tetrahydropiridine (MPTP) is a neurotoxin, widely used to produce experimental models of Parkinson Disease in rodents and primates. Although dopaminergic neurons are the most sensitive to MPTP neurotoxicity, different neuronal subtypes are affected. Among these, recent studies indicate that MPTP may produce pathological effects on spinal neurons. In fact, MPTP activates apoptotic proteins within the spinal cord and in particular within the motor neurons, suggesting commonalities between Parkinson Disease and Amyotrophic Lateral Sclerosis. In order to assess this point, in the present study we measured whether MPTP produces motor neurons loss. We chose a dose of MPTP (20 mg/kg × 3, 2 h apart), which in C57BL/6N mice was able to induce a massive nigrostriatal damage. Since both Parkinson Disease and Amyotrophic Lateral Sclerosis are characterized by altered alpha-synuclein immunostaining, this protein was also evaluated within spinal motor neurons, following MPTP administration. Three different monoclonal antibodies, recognizing distinct epitopes in the sequence of alpha-synuclein were used. Severe dopaminergic cell loss was quantified by stereology within the substantia nigra pars compacta, along with...Continue Reading

References

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Mar 12, 2011·Journal of Chemical Neuroanatomy·Giorgio VivacquaLoredana D'Este

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Citations

Jul 24, 2013·Journal of Neurochemistry·Supriti SamantarayNaren L Banik
Dec 18, 2013·Journal of Neurochemistry·Varduhi H KnaryanNaren L Banik
Sep 2, 2016·Cellular and Molecular Neurobiology·Guo-Ying FengTing-Hua Wang
Jun 27, 2017·Acta Pharmacologica Sinica·Qiu-Shuang ZhangNai-Hong Chen
Nov 14, 2019·Neurotoxicity Research·Giorgio VivacquaFrancesco Fornai
Nov 19, 2020·Neurotoxicity Research·Francesca BiagioniFrancesco Fornai

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