Loss of the anion exchanger DRA (Slc26a3), or PAT1 (Slc26a6), alters sulfate transport by the distal ileum and overall sulfate homeostasis

American Journal of Physiology. Gastrointestinal and Liver Physiology
Jonathan M Whittamore, Marguerite Hatch

Abstract

The ileum is considered the primary site of inorganic sulfate ([Formula: see text]) absorption. In the present study, we explored the contributions of the apical chloride/bicarbonate (Cl-/[Formula: see text]) exchangers downregulated in adenoma (DRA; Slc26a3), and putative anion transporter 1 (PAT1; Slc26a6), to the underlying transport mechanism. Transepithelial 35[Formula: see text] and 36Cl- fluxes were determined across isolated, short-circuited segments of the distal ileum from wild-type (WT), DRA-knockout (KO), and PAT1-KO mice, together with measurements of urine and plasma sulfate. The WT distal ileum supported net sulfate absorption [197.37 ± 13.61 (SE) nmol·cm-2·h-1], but neither DRA nor PAT1 directly contributed to the unidirectional mucosal-to-serosal flux ([Formula: see text]), which was sensitive to serosal (but not mucosal) DIDS, dependent on Cl-, and regulated by cAMP. However, the absence of DRA significantly enhanced net sulfate absorption by one-third via a simultaneous rise in [Formula: see text] and a 30% reduction to the secretory serosal-to-mucosal flux ([Formula: see text]). We propose that DRA, together with PAT1, contributes to [Formula: see text] by mediating sulfate efflux across the apical membrane....Continue Reading

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Citations

Nov 2, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jonathan M WhittamoreMarguerite Hatch
Jan 8, 2019·Experimental Physiology·Christine E StephensMarguerite Hatch
May 10, 2019·Animals : an Open Access Journal From MDPI·Micol BertocchiPaolo Trevisi
Aug 18, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Daniel R McHughCraig A Hodges
Jun 7, 2019·The Journal of Experimental Biology·Yoshio TakeiMasaaki Ando
Mar 14, 2018·The Journal of Biological Chemistry·Taro MukaiboJames E Melvin
Sep 19, 2019·American Journal of Physiology. Cell Physiology·Takashi MunemasaJames E Melvin
Nov 19, 2020·Pflügers Archiv : European journal of physiology·Jonathan M Whittamore, Marguerite Hatch

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Methods Mentioned

BETA
PCR
urine collections
urine collection

Software Mentioned

SigmaStat
SigmaPlot

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