Loss of the starvation-induced gene Rack1 leads to glycogen deficiency and impaired autophagic responses in Drosophila.

Autophagy
B ErdiGábor Juhász

Abstract

Autophagy delivers cytoplasmic material for lysosomal degradation in eukaryotic cells. Starvation induces high levels of autophagy to promote survival in the lack of nutrients. We compared genome-wide transcriptional profiles of fed and starved control, autophagy-deficient Atg7 and Atg1 null mutant Drosophila larvae to search for novel regulators of autophagy. Genes involved in catabolic processes including autophagy were transcriptionally upregulated in all cases. We also detected repression of genes involved in DNA replication in autophagy mutants compared with control animals. The expression of Rack1 (receptor of activated protein kinase C 1) increased 4.1- to 5.5-fold during nutrient deprivation in all three genotypes. The scaffold protein Rack1 plays a role in a wide range of processes including translation, cell adhesion and migration, cell survival and cancer. Loss of Rack1 led to attenuated autophagic response to starvation, and glycogen stores were decreased 11.8-fold in Rack1 mutant cells. Endogenous Rack1 partially colocalized with GFP-Atg8a and early autophagic structures on the ultrastructural level, suggesting its involvement in autophagosome formation. Endogenous Rack1 also showed a high degree of colocalization ...Continue Reading

Associated Datasets

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Citations

Mar 26, 2013·Acta Pharmacologica Sinica·Ya-ping YangChun-feng Liu
Jan 17, 2013·Cardiovascular Diabetology·Márta SárközyTamás Csont
Jun 21, 2014·BioMed Research International·Nitha C MulakkalIoannis P Nezis
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Feb 24, 2015·Frontiers in Neurology·Pankaj Kumar Singh, Sweta Singh
Feb 21, 2014·Molecular Biology of the Cell·Szabolcs TakátsGábor Juhász
Nov 20, 2018·Cell Death & Disease·Ta XiaoHong Yi
Apr 19, 2018·G3 : Genes - Genomes - Genetics·Amanda E CroftonSusan E Lott
Apr 23, 2020·PLoS Genetics·David BrooksErika R Geisbrecht
Jul 15, 2017·Cells·Péter LőrinczGábor Juhász
Nov 21, 2019·British Journal of Pharmacology·Li-Tao LiuLi-Hong Wan
Jun 22, 2016·The Journal of Biological Chemistry·Secil ErbilDevrim Gozuacik
Aug 10, 2019·Frontiers in Cell and Developmental Biology·Tamás MaruzsGábor Juhász
Jan 22, 2021·Developmental and Comparative Immunology·Samara Rosendo MachadoPascal Miesen

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Datasets Mentioned

BETA
E-MEXP-3352

Methods Mentioned

BETA
lipidation
transcription profiling
PCR
Transmission electron microscopy
transgenic
electron microscopy
confocal microscopy
co-immunoprecipitation
in vitro transcription
Feature Extraction

Software Mentioned

Feature Extraction
Adobe Photoshop
Agilent
MIAME
JColorGrid
Genspring
iTEM
BLASTP
Excel
DAVID Bioinformatics Resources

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