Loss of the tumor suppressor Pten promotes proliferation of Drosophila melanogaster cells in vitro and gives rise to continuous cell lines.

PloS One
Steven E JustinianoAmanda Simcox

Abstract

In vivo analysis of Drosophila melanogaster has enhanced our understanding of many biological processes, notably the mechanisms of heredity and development. While in vivo analysis of mutants has been a strength of the field, analyzing fly cells in culture is valuable for cell biological, biochemical and whole genome approaches in which large numbers of homogeneous cells are required. An efficient genetic method to derive Drosophila cell lines using expression of an oncogenic form of Ras (Ras(V12)) has been developed. Mutations in tumor suppressors, which are known to cause cell hyperproliferation in vivo, could provide another method for generating Drosophila cell lines. Here we screened Drosophila tumor suppressor mutations to test if they promoted cell proliferation in vitro. We generated primary cultures and determined when patches of proliferating cells first emerged. These cells emerged on average at 37 days in wild-type cultures. Using this assay we found that a Pten mutation had a strong effect. Patches of proliferating cells appeared on average at 11 days and the cultures became confluent in about 3 weeks, which is similar to the timeframe for cultures expressing Ras(V12). Three Pten mutant cell lines were generated and...Continue Reading

References

Apr 1, 1986·Journal of the National Cancer Institute·P M KraemerL S Cram
May 1, 1972·Thorax·M R KeighleyM I Ionescu
Sep 5, 2002·Genes & Development·David A Prober, Bruce A Edgar
Nov 15, 2002·The New England Journal of Medicine·William C Hahn, Robert A Weinberg
Jul 22, 2005·Nature Reviews. Cancer·Anthony M Brumby, Helena E Richardson
Jul 29, 2006·Annual Review of Genetics·Iswar K Hariharan, David Bilder
Nov 26, 2008·Oncogene·P O HumbertH E Richardson
Dec 17, 2008·Fly·Amanda A SimcoxHamed Jafar-Nejad
Apr 7, 2010·Current Biology : CB·Brian S RobinsonKenneth H Moberg
Apr 7, 2010·Current Biology : CB·Nicola A GrzeschikHelena E Richardson
May 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chen LingDuojia Pan
Jun 30, 2010·PLoS Genetics·Lutz KockelNorbert Perrimon
Aug 28, 2010·Fly·Linda M ParsonsHelena E Richardson
Oct 19, 2010·Developmental Cell·Duojia Pan
Dec 24, 2010·Genome Research·Lucy CherbasPeter Cherbas
Aug 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mary-Lou Pardue, P G DeBaryshe

❮ Previous
Next ❯

Citations

Mar 17, 2015·FEBS Letters·Teri HatzihristidisDiego Miranda-Saavedra
Jan 18, 2014·Methods : a Companion to Methods in Enzymology·Lucy Cherbas, Lei Gong
Dec 19, 2018·Wiley Interdisciplinary Reviews. Developmental Biology·Arthur LuhurAndrew C Zelhof
Jan 17, 2016·G3 : Genes - Genomes - Genetics·Marcus StoiberPeter Cherbas
Oct 9, 2020·G3 : Genes - Genomes - Genetics·Arthur LuhurAndrew C Zelhof

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections
N J WeismanM D Golubovsky
Nature Reviews. Cancer
Nicola McCarthy
Nature Reviews. Molecular Cell Biology
Nicola McCarthy
© 2022 Meta ULC. All rights reserved